Aytekin Alper, Kadakal Hikmet, Mihcioglu Deniz, Gurer Turkan
Department of General Surgery, Faculty of Medicine, Gaziantep University, Gaziantep, 27310, Turkey.
Department of Biology, Faculty of Art and Science, Gaziantep University, Gaziantep, 27310, Turkey.
BMC Med Genomics. 2025 Jan 2;18(1):1. doi: 10.1186/s12920-024-02080-6.
The study aimed to was to investigate the relationship between miR-2861, miR-5011-5p, and colorectal carcinogenesis.
In the present study, it was isolated RNA from both the tumor and non-tumor tissue of a total of 80 CRC patients and after synthesizing the cDNA, it was performed qRT-PCR to determine the expression levels of miR‑2861 and miR‑5011-5p. In addition, it was predicted that dysregulated miRNAs targets, pathways and functional gene annotations that may be important in colorectal carcinogenesis using KEGG pathway and GO analysis.
The resulting data revealed that both expression levels of miR-2861 and miR-5011-5p were significantly decreased in tumor tissues compared with non-tumor tissues of CRC patients. The GO and KEGG pathway analysis showed that miR-2861 and miR-5011-5p may participate in multiple the biological process, cellular components, and molecular function subcategories such as mitotic cell cycle, regulation of small GTPase mediated signal transduction, cell death, and acid binding transcription factor activity. It was also revealed that target genes of miRNAs can be found in signaling pathways such as TGF-beta, Rap1, Ras, cAMP, Wnt, mTOR and, PI3K-Akt signaling pathways.
These findings imply that miR-2861 and miR-5011-5p might function as tumor suppressors in the development of CRC.
本研究旨在探讨miR-2861、miR-5011-5p与结直肠癌发生之间的关系。
在本研究中，从总共80例结直肠癌患者的肿瘤组织和非肿瘤组织中分离RNA，合成cDNA后，进行qRT-PCR以测定miR-2861和miR-5011-5p的表达水平。此外，使用KEGG通路和GO分析预测在结直肠癌发生中可能重要的失调miRNA靶标、途径和功能基因注释。
所得数据显示，与结直肠癌患者的非肿瘤组织相比，肿瘤组织中miR-2861和miR-5011-5p的表达水平均显著降低。GO和KEGG通路分析表明，miR-2861和miR-5011-5p可能参与多个生物学过程、细胞成分和分子功能亚类，如细胞有丝分裂周期、小GTPase介导的信号转导调节、细胞死亡和酸性结合转录因子活性。还发现miRNA的靶基因存在于TGF-β、Rap1、Ras、cAMP、Wnt、mTOR和PI3K-Akt信号通路等信号通路中。
这些发现表明，miR-2861和miR-5011-5p在结直肠癌的发生发展中可能起肿瘤抑制作用。
