分析 miR-143、miR-1、miR-210 和 let-7e 在结直肠癌中与组织病理学特征的关系。
Analysis of miR-143, miR-1, miR-210 and let-7e Expression in Colorectal Cancer in Relation to Histopathological Features.
机构信息
Laboratory of Cancer Genetics, Department of Pathology, Polish Mother's Memorial Hospital Research Institute, Rzgowska 281/289, 93-338 Lodz, Poland.
Department of General and Transplant Surgery, N. Barlicki Memorial Clinical Hospital, Medical University of Lodz, 90-153 Lodz, Poland.
出版信息
Genes (Basel). 2022 May 13;13(5):875. doi: 10.3390/genes13050875.
BACKGROUND
MicroRNAs (miRNAs) are small RNA molecules involved in the control of the expression of many genes and are responsible for, among other things, cell death, differentiation and the control of their division. Changes in miRNA expression profiles have been observed in colorectal cancer. This discovery significantly enriches our knowledge of the pathogenesis of colorectal cancer and offers new goals in diagnostics and therapy.
AIM
The aim of this study was to analyze the expression of four miRNA sequences-miR-143, miR-1, miR-210 and let-7e-and to investigate their significance in the risk of developing colorectal cancer.
MATERIALS AND METHODS
miRNA sequences were investigated in formalin-fixed, paraffin-embedded (FFPE) tissue in colorectal cancer patients ( = 150) and in cancer-free controls ( = 150). The real-time PCR method was used.
RESULTS
This study revealed a lower expression of miR-143 in colorectal cancer patients than in the controls. miR-143 was positively correlated with the degree of tumor differentiation (grading). Three out of four analyzed miRNA (miR-1, miR-210 and let-7e) were found to be statistically insignificant in terms of colorectal carcinoma risk.
CONCLUSIONS
miR-143 may be associated with the development of colorectal cancer.
背景
微小 RNA（miRNAs）是参与调控许多基因表达的小 RNA 分子，除其他功能外，还负责细胞死亡、分化和控制其分裂。在结直肠癌中观察到 miRNA 表达谱的变化。这一发现极大地丰富了我们对结直肠癌发病机制的认识，并为诊断和治疗提供了新的目标。
目的
本研究旨在分析四种 miRNA 序列（miR-143、miR-1、miR-210 和 let-7e）的表达情况，并探讨它们在结直肠癌发病风险中的意义。
材料与方法
在结直肠癌患者（n=150）和无癌对照者（n=150）的福尔马林固定、石蜡包埋（FFPE）组织中研究了 miRNA 序列。采用实时 PCR 法。
结果
本研究表明，结直肠癌患者 miR-143 的表达低于对照组。miR-143 与肿瘤分化程度（分级）呈正相关。在分析的四种 miRNA（miR-1、miR-210 和 let-7e）中，有三种在结直肠癌风险方面无统计学意义。
结论
miR-143 可能与结直肠癌的发生有关。
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