Saenchoopa Apichart, Plaeyao Kittiya, Talodthaisong Chanon, Thet Tun Wonn Shweyi, Nasomjai Pitak, Lapmanee Sarawut, Somsakeesit La-Or, Hutchison James A, Kulchat Sirinan
Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Department of System Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Macromol Biosci. 2025 Apr;25(4):e2400504. doi: 10.1002/mabi.202400504. Epub 2025 Jan 2.
To address the rising prevalence of bacterial infections and the need for innovative therapeutic solutions, this study has developed a novel antibacterial hydrogel composite composed of Aloe vera, gelatin, sodium alginate, and Sterculia monosperma-silver nanoparticles (SM-AgNPs) loaded curcumin-nanoliposomes (NLPs). The aloe vera/gelatin/sodium alginate hydrogels (AGS) are prepared using different weight ratios of Aloe vera, gelatin, and sodium alginate, aiming to optimize mechanical properties and biocompatibility for biomedical applications. The incorporation of SM-AgNPs and curcumin-loaded NLPs enhanced the hydrogels' antibacterial properties. Characterizations of the hydrogels are performed by using Fourier-transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. Additional examinations, such as water absorption analysis, rheology measurements, thermal stability, and injectability, along with pH and temperature responsiveness, are also conducted. The AGS-3 hydrogel formulation, with a 1:5:3 ratio of Aloe vera to gelatin to sodium alginate, exhibited significant performance in all tests, making it suitable for further experiments. Furthermore, antimicrobial activity assays showed that AGS hydrogels containing SM-AgNPs/NLP composites effectively inhibited the growth of both gram-positive Staphylococcus aureus (S.aureus) and gram-negative Escherichia coli (E.coli) bacteria. These results indicate that the SM-AgNPs/NLP-AGS hydrogel is a promising material for biomedical applications including wound healing, infection prevention, and targeted drug delivery.
为应对细菌感染患病率不断上升的情况以及对创新治疗解决方案的需求，本研究开发了一种新型抗菌水凝胶复合材料，其由芦荟、明胶、海藻酸钠以及负载有姜黄素纳米脂质体（NLPs）的苹婆属单籽银纳米颗粒（SM-AgNPs）组成。芦荟/明胶/海藻酸钠水凝胶（AGS）是使用芦荟、明胶和海藻酸钠的不同重量比制备的，旨在优化其力学性能和生物相容性以用于生物医学应用。SM-AgNPs和负载姜黄素的NLPs的加入增强了水凝胶的抗菌性能。通过傅里叶变换红外光谱、热重分析和扫描电子显微镜对水凝胶进行表征。还进行了其他检测，如水吸收分析、流变学测量、热稳定性和可注射性，以及pH和温度响应性检测。芦荟与明胶与海藻酸钠比例为1:5:3的AGS-3水凝胶配方在所有测试中均表现出显著性能，使其适合进一步实验。此外，抗菌活性测定表明，含有SM-AgNPs/NLP复合材料的AGS水凝胶能有效抑制革兰氏阳性金黄色葡萄球菌（S.aureus）和革兰氏阴性大肠杆菌（E.coli）的生长。这些结果表明，SM-AgNPs/NLP-AGS水凝胶是一种有前途的材料，可用于生物医学应用，包括伤口愈合、感染预防和靶向药物递送。
