Shokri Pourya, Tabatabaei Shahin, Ziaee Seyed Amir Mohsen, Pedram Mir Sepehr, Dehghan Mohammad Mehdi, Shakhssalim Nasser
Urology and Nephrology Research Center, Research Institute of Urology and Nephrology, Shahid Labbafinejad Medical Center, Urology Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Anesthesiology Research Center, Anesthesiology Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Animal Model Exp Med. 2025 Feb;8(2):372-377. doi: 10.1002/ame2.12519. Epub 2025 Jan 3.
This study developed an animal model with internal and external urethral sphincter insufficiency by bypassing the sphincter without major damage so that the animal under study can return to normal life after the study. There is a need for a reliable, applicable, and reproducible animal model for studying urinary incontinency disease due to incorrect sphincter function. Seven adult male dogs were used for this study. The urethral sphincter was bypassed by inserting a catheter between the bladder neck and the distal sphincter. The animals' physical condition was closely monitored for 9 weeks, and standard urodynamic and radiologic studies were performed before and 1-2 months after surgery. The animals were killed at 9 weeks after surgery for pathological assessment. Catheter placement caused complete incontinence in the animal, with urodynamic assessments indicating that the animal was unable to control urination and radiological assessments indicating an empty bladder with a residual volume of 50 ± 10 cc. Tissue analysis did not show significant histological damage and inflammation. The study shows that by bypassing the urethral sphincter, which is a reliable and reproducible method, an animal model of urinary incontinence can be developed, which can be used in various studies such as assessing the adequacy of artificial sphincter function. The animals under study did not have any permanent defect, so they were able to return to their normal life.
本研究通过在不造成严重损伤的情况下绕过尿道括约肌，建立了一种内外尿道括约肌功能不全的动物模型，以便受试动物在研究结束后能够恢复正常生活。由于括约肌功能异常导致尿失禁疾病的研究需要一种可靠、适用且可重复的动物模型。本研究使用了7只成年雄性犬。通过在膀胱颈和远端括约肌之间插入导管来绕过尿道括约肌。对动物的身体状况进行了9周的密切监测，并在手术前以及手术后1 - 2个月进行了标准的尿动力学和放射学研究。在手术后9周处死动物进行病理评估。导管置入导致动物完全失禁，尿动力学评估表明动物无法控制排尿，放射学评估显示膀胱空虚，残余尿量为50±10立方厘米。组织分析未显示明显的组织学损伤和炎症。该研究表明，通过绕过尿道括约肌这一可靠且可重复的方法，可以建立尿失禁动物模型，该模型可用于各种研究，如评估人工括约肌功能的充分性。受试动物没有任何永久性缺陷，因此能够恢复正常生活。
