Guła Zofia, Krzyściak Wirginia, Kuszmiersz Piotr, Bystrowska Beata, Korkosz Mariusz
Department of Rheumatology and Immunology, Jagiellonian University Medical College, Jakubowskiego 2, Kraków, 30-688, Poland.
Department of Rheumatology, Clinical Immunology and Internal Medicine, University Hospital in Cracow, Kraków, 30-688, Poland.
Rheumatol Int. 2025 Jan 3;45(1):17. doi: 10.1007/s00296-024-05769-0.
Growing evidence suggests that serotonin is an important mediator in the cross-talk between immune and bone cells, playing a role in the pathogenesis of various types of inflammatory arthritis (IA). However, the relationship between circulating serotonin and different outcomes in three most prevalent IA - rheumatoid arthritis (RA), psoriatic arthritis (PsA), and axial spondyloarthritis (axSpA), remains limited and requires further investigation. This study was performed to evaluate variations in serotonin serum levels among RA, PsA, and axSpA and to explore the utility of this biochemical marker in the assessment of disease activity and health status measurements provided by the Multi-Dimensional Health Assessment Questionnaire (MDHAQ). This was a cross-sectional study using data from the PolNorRHEUMA registry. Demographic and clinical data, as well as blood samples, were collected during routine visits to the rheumatology outpatient clinic. We included 60 patients (20 with RA, 20 with PsA, and 20 with axSpA) and 45 healthy controls, with a mean age of 49 years and 56.2% female. A reliable liquid chromatography-tandem mass spectrometry (LC-MS) method was used for the quantitative determination of serotonin in blood serum. Analysis of serotonin levels, based on 105 observations and adjusted for age, SSRI/SNRI intake and physical activity, revealed a significant elevation in the patient groups compared with the controls (p < 0.001): 134.00 ng/mL in healthy controls vs. 176.00 ng/mL in RA, 183 ng/mL in PsA, and 184.00 ng/mL in axSpA, with no statistically significant differences between the respective forms of IA. We found no significant correlation between the serotonin concentration and disease activity composite scores. A sample of 51 patients revealed a significant positive correlation between the serotonin concentration and global MDHAQ scores (β = 0.01, p = 0.009), indicating that an increase in serotonin levels is associated with worsening patient-reported health status. The serotonin serum concentration was higher in patients with RA, PsA, and axSpA than in controls, indicating its potential as a biomarker of inflammation and worse health status. The LC-MS method was successfully applied for the analysis of serum.
越来越多的证据表明，血清素是免疫细胞与骨细胞之间相互作用的重要介质，在各种类型的炎症性关节炎（IA）的发病机制中发挥作用。然而，循环血清素与三种最常见的IA——类风湿性关节炎（RA）、银屑病关节炎（PsA）和轴性脊柱关节炎（axSpA）的不同结局之间的关系仍然有限，需要进一步研究。本研究旨在评估RA、PsA和axSpA患者血清素水平的变化，并探讨这种生化标志物在评估疾病活动度以及多维健康评估问卷（MDHAQ）所提供的健康状况测量中的效用。这是一项利用PolNorRHEUMA注册中心数据的横断面研究。在风湿科门诊的常规就诊期间收集人口统计学和临床数据以及血样。我们纳入了60例患者（20例RA患者、20例PsA患者和20例axSpA患者）以及45名健康对照者，平均年龄为49岁，女性占56.2%。采用可靠的液相色谱 - 串联质谱（LC - MS）方法对血清中的血清素进行定量测定。基于105次观察结果并对年龄、SSRI/SNRI摄入量和身体活动进行校正后，血清素水平分析显示，与对照组相比，患者组血清素水平显著升高（p < 0.001）：健康对照组为134.00 ng/mL，RA患者组为176.00 ng/mL，PsA患者组为183 ng/mL，axSpA患者组为184.00 ng/mL，各型IA之间无统计学显著差异。我们发现血清素浓度与疾病活动度综合评分之间无显著相关性。对51例患者的样本分析显示，血清素浓度与MDHAQ总体评分之间存在显著正相关（β = 0.01，p = 0.009），这表明血清素水平升高与患者报告的健康状况恶化相关。RA、PsA和axSpA患者的血清血清素浓度高于对照组，表明其有潜力作为炎症和较差健康状况的生物标志物。LC - MS方法成功应用于血清分析。
