Bagale Siddharam Shivappa, Deshmukh Priyanka U, Mandal Soumyadeep, Malvi Harshada, Kondabagil Kiran, Pradeepkumar P I
Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
J Org Chem. 2025 Jan 17;90(2):1159-1166. doi: 10.1021/acs.joc.4c01683. Epub 2025 Jan 3.
The DNA adducts formed by the alkenylbenzene natural products, safrole (SF) and methyleugenol (MEG) are primarily attributed to their reported carcinogenic properties. Herein, we report a concise strategy to access -Ac-SF/MEG-dA phosphoramidites, which were selectively incorporated into DNA oligonucleotides by solid-phase DNA synthesis. The replication studies using human polymerases hpolκ and hpolη showed that both polymerases replicate these adducts error-free, which indicates that these polymerases do not contribute to the adduct-induced mutagenicity.
由链烯基苯天然产物黄樟素（SF）和甲基丁香酚（MEG）形成的DNA加合物主要归因于它们已报道的致癌特性。在此，我们报告了一种简洁的策略来获得 -Ac-SF/MEG-dA亚磷酰胺，它们通过固相DNA合成被选择性地掺入DNA寡核苷酸中。使用人聚合酶hpolκ和hpolη进行的复制研究表明，这两种聚合酶都能无错误地复制这些加合物，这表明这些聚合酶不会导致加合物诱导的致突变性。
