Liu Jiayu, Kuang Jialiang, Chen Xiangwen, Huang Linan, Shi Zhenqing
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou, Guangdong 510006, PR China.
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou, Guangdong 510006, PR China.
J Hazard Mater. 2025 Mar 15;486:137027. doi: 10.1016/j.jhazmat.2024.137027. Epub 2024 Dec 26.
Microbe-mediated remediation becomes a desire method for removal of persistent organic pollutants (POPs) due to its eco-friendly and sustainable nature. The improvement of practical feasibility requires constructing comprehensive species pool, while it is still limited by the rapid recognition of potential bacterial resources from environment. Here, based on the relative abundances of bacterial OTUs and pollutant concentrations, we established indexes to assess their tolerance to organochlorine pesticides (OCPs) and flame retardants (FRs) that are atmospheric transported and naturally accumulated in forest soil via forest filter effect. By exploring the tolerance pattern from tropical to temperate forests across China, we demonstrated that diversity, community composition, and relative abundances of POP-tolerant bacteria were significantly related to POPs' distribution and concentration. By recognizing over a hundred of genera composed of POP-tolerant species, we found that OCP-tolerant taxa were wide-distributed, while bacterial communities were more responsive to the contamination level of FRs and FR-tolerant taxa were accumulated along the increase of FRs pollution. Our indexes successfully recognized well-known POPs-degrading genera, including Rhodococcus, Bacillus, Arthrobacter, Stenotrophomonas, and Pseudomonas, as well as a series of versatile taxa affiliated with families Ktedonobacteraceae, Acetobacteraceae, Solirubrobacteraceae, and Nocardiaceae, which were extremely rare and likely ignored in laboratory-scale experiments. Together, our findings provide valuable clues to expand the library of POPs-degrading candidates that is helpful in screening bacterial resources for bioremediation.
微生物介导的修复因其生态友好和可持续的特性，成为去除持久性有机污染物（POPs）的理想方法。提高实际可行性需要构建全面的物种库，然而，从环境中快速识别潜在细菌资源仍然受到限制。在此，基于细菌OTU的相对丰度和污染物浓度，我们建立了指标，以评估它们对通过森林过滤效应在森林土壤中大气传输和自然积累的有机氯农药（OCPs）和阻燃剂（FRs）的耐受性。通过探索中国从热带到温带森林的耐受性模式，我们证明了耐POPs细菌的多样性、群落组成和相对丰度与POPs的分布和浓度显著相关。通过识别由耐POPs物种组成的一百多个属，我们发现耐OCPs分类群分布广泛，而细菌群落对FRs的污染水平更敏感，并且耐FRs分类群随着FRs污染的增加而积累。我们的指标成功识别了著名的POPs降解属，包括红球菌属、芽孢杆菌属、节杆菌属、嗜麦芽窄食单胞菌属和假单胞菌属，以及一系列与凯特杆菌科、醋酸杆菌科、红嗜热放线菌科和诺卡氏菌科相关的多功能分类群，这些分类群在实验室规模的实验中极为罕见且可能被忽视。总之，我们的研究结果为扩展POPs降解候选库提供了有价值的线索，这有助于筛选用于生物修复的细菌资源。
