Qi Junjie, Wan Yuqi, Li Jingkun, Jiang Guoyong, Wang Jiahua, Ozaki Yukihiro, Pi Fuwei
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China; International Joint Laboratory on Food Safety, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, People's Republic of China.
Biosens Bioelectron. 2025 Mar 15;272:117114. doi: 10.1016/j.bios.2024.117114. Epub 2024 Dec 30.
Tetracycline antibiotics (TCs) are extensively used as broad-spectrum antimicrobials. However, their excessive use and misuse have led to serious accumulation in foods and environments, posing a significant threat to human health. To solve such public issue, we have designed a novel dual-mode detection method, integrating colorimetric sensing with surface-enhanced Raman scattering (SERS) technology, for sensitive and rapid evaluation on TCs. In this method, an Ag@NH-MIL-101(Al)-Ag nanoprobe that leverages the synergistic effect between silver nanoparticles (AgNPs) and NH-MIL-101(Al), resulting in remarkable peroxidase-like activity and enhanced SERS performance, was proposed. Moreover, for the instant colorimetric sensing, a competitive oxidative-reaction between TCs and 3,3',5,5'-tetramethylbenzidine (TMB) was developed based on the peroxidase-like activity of nanoprobe. Our innovative colorimetric-SERS dual-mode approach demonstrated high sensitivity accompanied by robust selectivity with limits of detection (LODs) for TCs of 10 ppm in colorimetric mode and 10 ppm in SERS mode. Furthermore, TCs can be reliably detected in honey samples with recoveries ranging from 87.69% to 120.49%. This dual-mode sensing strategy effectively combining the ease of colorimetric detection with high sensitivity of SERS provides significant values for rapid, direct, and multiplexed evaluation on various hazards in environment and food chains.
四环素类抗生素（TCs）被广泛用作广谱抗菌剂。然而，它们的过度使用和滥用导致其在食品和环境中严重累积，对人类健康构成重大威胁。为了解决这一公共问题，我们设计了一种新型的双模式检测方法，将比色传感与表面增强拉曼散射（SERS）技术相结合，用于对TCs进行灵敏且快速的评估。在该方法中，提出了一种Ag@NH-MIL-101(Al)-Ag纳米探针，它利用了银纳米颗粒（AgNPs）与NH-MIL-101(Al)之间的协同效应，产生了显著的类过氧化物酶活性并增强了SERS性能。此外，对于即时比色传感，基于纳米探针的类过氧化物酶活性，开发了TCs与3,3',5,5'-四甲基联苯胺（TMB）之间的竞争性氧化反应。我们创新的比色-SERS双模式方法表现出高灵敏度以及强大的选择性，TCs在比色模式下的检测限（LOD）为10 ppm，在SERS模式下为10 ppm。此外，在蜂蜜样品中能够可靠地检测到TCs，回收率在87.69%至120.49%之间。这种将比色检测的简便性与SERS的高灵敏度有效结合的双模式传感策略，对于快速、直接和多重评估环境和食物链中的各种危害具有重要价值。
