State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
Food Chem. 2024 Mar 1;435:137586. doi: 10.1016/j.foodchem.2023.137586. Epub 2023 Sep 24.
Antibiotic residues are becoming more and more concern due to the increasingly serious resistance from bacteria to organism. On-site and accurate evaluation on antibiotics is necessary and urgent to effectively solve such public issue. To provide point-of-care-test (POCT) ideas for antibiotic accurate evaluation, a fluorescence (FL)-surface-enhanced Raman scattering (SERS) dual-mode detection of tetracycline antibiotic (TCs) was realized for the first time. Based on the inner filter effect in Ag@NH-MIL-101(Al) nanoprobe, the fluorescence quenching was induced and the SERS signal was swiftly turn on through π-π interaction and hydrogen bonding in the presence of TCs. This FL-SERS dual mode sensor displayed excellent detection limits (FL in ∼10 ppm, SERS in ∼10 ppm), and achieved a reliable detection of TCs in honey with a recovery rate of 84.45%-112.08%. This method combines the advantages of FL and SERS detection, meanwhile, two techniques verified against each other to achieve highly sensitive and specific FL-SERS dual-mode sensor for TCs. We believe that such antibody-or aptamer-independent FL and SERS complementary nanoprobe can be applied to fast, direct and multiple sensing in environment and food hazards.
由于细菌对生物体的耐药性越来越严重，抗生素残留问题越来越受到关注。现场、准确地评价抗生素对于有效解决此类公共问题是必要且紧迫的。为了为抗生素的准确评价提供即时检测（POCT）思路，首次实现了四环素抗生素（TCs）的荧光（FL）-表面增强拉曼散射（SERS）双模检测。基于 Ag@NH-MIL-101（Al）纳米探针中的内滤效应，在 TCs 的存在下，通过π-π 相互作用和氢键诱导荧光猝灭，并迅速开启 SERS 信号。这种 FL-SERS 双模传感器表现出优异的检测限（FL 在10 ppm，SERS 在10 ppm），并实现了蜂蜜中 TCs 的可靠检测，回收率为 84.45%-112.08%。该方法结合了 FL 和 SERS 检测的优点，同时，两种技术相互验证，实现了高灵敏度和特异性的 FL-SERS 双模传感器用于 TCs。我们相信，这种抗体或适配体独立的 FL 和 SERS 互补纳米探针可应用于环境和食品安全危害的快速、直接和多重传感。
