Ruifrok A C, Kanon B, Konings A W
Radiat Res. 1985 Feb;101(2):326-31.
Mouse fibroblast LM cells have been heated at 44 degrees C for different periods. Potassium content of the cells was measured at certain intervals during the postheating period at 37 degrees C for up to 24 hr. The level of K+ decreased gradually in time starting within some hours after the heat treatment. The rate of K+ loss as well as the ultimate level reached was heat-dose dependent. When the potassium content of the cell population was determined 16 hr after the heat treatment, a correlation was observed between the concentration of potassium and the level of cell survival. When X irradiation was applied immediately after hyperthermia, radiosensitization on the level of cell survival was obtained as expected, the extent being dependent on the severity of heat treatments. No added K+ loss was observed, however, when hyperthermia was combined with radiation. It is suggested that plasma membrane related functions are disturbed by the heat treatment. This points to membranes as possible candidates for primary targets in the case of cell inactivation by heat alone, and not with respect to the radiosensitization by hyperthermia.
小鼠成纤维细胞LM细胞在44摄氏度下加热不同时长。在37摄氏度的加热后阶段，每隔一定时间测量细胞的钾含量，最长测量24小时。热处理后数小时内，钾离子水平开始随时间逐渐下降。钾离子流失速率以及最终达到的水平取决于热剂量。热处理16小时后测定细胞群体的钾含量时，观察到钾浓度与细胞存活水平之间存在相关性。热疗后立即进行X射线照射，如预期那样在细胞存活水平上获得了放射增敏作用，其程度取决于热处理的强度。然而，当热疗与辐射联合使用时，未观察到额外的钾离子流失。这表明热处理会干扰与质膜相关的功能。这表明在仅通过热使细胞失活的情况下，质膜可能是主要靶点的候选者，而不是在热疗放射增敏方面。
