Louglali Fadoua, Jaafari Abdeslam, Lekchiri Souad, Taoufik Hakim, Zanane Chorouk, Meftah Iman, Liba Ahmed, El Louali Mostafa, Zahir Hafida, Latrache Hassan
Industrial and Surface Engineering Laboratory, Bioprocess and Biointerfaces Team, Department of Life Sciences, Faculty of Sciences and Techniques, Sultan Moulay Slimane University, BP 523, 23000, Beni Mellal, Morocco.
Material Physics Laboratory, Faculty of Sciences and Techniques, Sultan Moulay Slimane University, BP 523, 23000, Beni Mellal, Morocco.
Curr Microbiol. 2025 Jan 3;82(2):67. doi: 10.1007/s00284-024-04052-6.
Salmonella Typhi can adhere to and build biofilms on the surface of gallstones causing abnormal gallbladder mucosa, which could lead to carcinogenesis. The surface physicochemical properties of microbial cells and materials have been shown to play a crucial role in adhesion. Therefore, the purpose of this study was to investigate, for the first time, the surface properties of nine gallstones and to evaluate the influence of these parameters on the theoretical adhesion of S. Typhi to gallstone surfaces. The physicochemical properties were determined by SEM-EDX and contact angle measurements (CAM) while the predictive adhesion of S. Typhi on gallstones was estimated using the XDLVO approach. SEM-EDX analysis revealed that cholesterol is the principal component on the surface of all gallstones, with carbon and oxygen as the main elements. Aluminum was detected as a trace element in only three gallstones: GS2, GS4, and GS5. S. Typhi CIP5535 has a hydrophilic character (ΔG = 33.54 mJ m), as well as strong electron donor (γ = 55,80 mJ m) and weak electron acceptor properties (γ = 1,95 mJ m). Regarding gallstones, it was found that they have a hydrophobic character (ΔG between -29,9 mJ m and -75,2 mJ m), while their electron donor/acceptor characters change according to each gallstone. Predictive adhesion showed that all gallstones could be colonized by S. Typhi except GS1, GS5, and GS6 . Understanding the interfacial phenomena implicated in the process of bacterial adhesion makes it possible to limit or even inhibit the adhesion of S. Typhi on gallstone surfaces.
伤寒沙门氏菌可附着于胆结石表面并形成生物膜，导致胆囊黏膜异常，进而可能引发癌变。微生物细胞和材料的表面物理化学性质已被证明在黏附中起着关键作用。因此，本研究的目的是首次研究九种胆结石的表面性质，并评估这些参数对伤寒沙门氏菌在胆结石表面理论黏附的影响。通过扫描电子显微镜-能谱仪（SEM-EDX）和接触角测量（CAM）来测定物理化学性质，同时使用扩展DLVO（XDLVO）方法估算伤寒沙门氏菌在胆结石上的预测黏附力。SEM-EDX分析表明，胆固醇是所有胆结石表面的主要成分，碳和氧是主要元素。仅在三种胆结石（GS2、GS4和GS5）中检测到铝作为微量元素。伤寒沙门氏菌CIP5535具有亲水性（ΔG = 33.54 mJ/m²），以及较强的电子供体性质（γ = 55.80 mJ/m²）和较弱的电子受体性质（γ = 1.95 mJ/m²）。关于胆结石，发现它们具有疏水性（ΔG在-29.9 mJ/m²至-75.2 mJ/m²之间），而它们的电子供体/受体性质因每种胆结石而异。预测黏附力表明，除了GS1、GS5和GS6之外，所有胆结石都可能被伤寒沙门氏菌定植。了解细菌黏附过程中涉及的界面现象使得限制甚至抑制伤寒沙门氏菌在胆结石表面的黏附成为可能。
