Zhang Junli, Zhao Yanyan, Hu Haiying, Lv Min, Zhang Hongling
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, Zhengzhou, 450001, China.
Mikrochim Acta. 2025 Jan 3;192(1):53. doi: 10.1007/s00604-024-06862-8.
A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×10 particles/mL and a linear range from 10 to 10 particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer's desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.
提出了一种纳米酶夹心测定法（SWzyme测定法），这是一种基于生物芯片和无机纳米酶的比色系统，用于快速、简单地测定血浆中的外泌体Aβ42。制备了抗CD63抗体修饰的生物芯片用于捕获血浆外泌体，并合成了用于检测外泌体Aβ42的高催化活性Ni@Pt纳米酶。该方法能够检测外泌体Aβ42，检测限（LOD）低至4.2×10颗粒/毫升，线性范围为10至10颗粒/毫升。通过测定外泌体Aβ42，SWzyme测定法成功区分了阿尔茨海默病（AD）小鼠和健康小鼠的血浆。SWzyme测定法有望成为AD早期检测的诊断工具，并支持个性化医学的发展。
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