Anhui Province Key Laboratory of Value-Added Catalytic Conversion and Reaction Engineering, Anhui Province Engineering Research Center of Flexible and Intelligent Materials School of Chemistry and Chemical Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, China.
Anhui Province Key Laboratory of Value-Added Catalytic Conversion and Reaction Engineering, Anhui Province Engineering Research Center of Flexible and Intelligent Materials School of Chemistry and Chemical Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, China.
Anal Chim Acta. 2024 Apr 22;1299:342453. doi: 10.1016/j.aca.2024.342453. Epub 2024 Mar 5.
The development of wearable detection devices that can achieve noninvasive, on-site and real-time monitoring of sweat metabolites is of great demand and practical significance for point-of-care testing and healthcare monitoring. Monitoring uric acid (UA) content in sweat provides a simple and promising way to reduce the risk of gout and hyperuricemia. Traditional bioenzyme based UA assays suffer from high cost, poor stability, inconvenience for storage and easy deactivation of bioenzymes. Wearable microfluidic colorimetric detection device for sweat UA detection has not been reported. The development of novel wearable microfluidic colorimetric detection chip with no requirement of bioenzymes for sweat UA detection is of great importance for health care monitoring.
Firstly, Co@MnO nanozyme with high oxidase-like activity was synthesized and characterized. Co@MnO can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) directly to generate blue-green colored ox-TMB. Green colored 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical (ABTS) was produced by the oxidation of ABTS by potassium persulfate. UA exhibits distinct quenching effect on Co@MnO catalyzed TMB colorimetric reaction system and ABTS based colorimetric system, leading to obvious color fading of the two colorimetric systems. Then, a flexible microfluidic colorimetric detection chip for UA detection was fabricated by assembling Co@MnO/TMB modified paper chips and ABTS modified paper chips into a polydimethylsiloxane (PDMS) microfluidic chip. The fabricated microfluidic colorimetric detection chip exhibits good linear relationship for sweat UA detection. The linear range is from 20 to 200 μmol/L with detection limit as low as 6.6 μmol/L. Good results were obtained for the detection of UA in actual sweat from three volunteers.
This work provides two bio-enzyme free colorimetric detection systems for UA detection. Furthermore, a simple, low-cost and selective flexible wearable microfluidic colorimetric detection chip was fabricated for noninvasive and on-site detection of sweat UA, which holds great application potential for personal health monitoring and point-of-care testing.
开发能够实现非侵入式、现场实时监测汗液代谢物的可穿戴检测设备，对于即时检测和医疗保健监测具有重要的需求和实际意义。监测汗液中的尿酸（UA）含量为降低痛风和高尿酸血症的风险提供了一种简单而有前途的方法。传统的基于生物酶的 UA 检测方法存在成本高、稳定性差、储存不便和生物酶易失活等问题。用于汗液 UA 检测的新型无酶可穿戴微流控比色检测装置尚未见报道。开发新型无酶可穿戴微流控比色检测芯片用于汗液 UA 检测，对于医疗保健监测具有重要意义。
首先，合成并表征了具有高过氧化物酶样活性的 Co@MnO 纳米酶。Co@MnO 可以直接催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化生成蓝色绿色的氧化 TMB。过硫酸钾氧化 ABTS 生成绿色的 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）自由基（ABTS）。UA 对 Co@MnO 催化的 TMB 比色反应体系和 ABTS 比色体系表现出明显的猝灭效应，导致两个比色体系的颜色明显褪色。然后，通过将 Co@MnO/TMB 修饰的纸芯片和 ABTS 修饰的纸芯片组装到聚二甲基硅氧烷（PDMS）微流控芯片中，制备了用于 UA 检测的柔性微流控比色检测芯片。所制备的微流控比色检测芯片对汗液 UA 检测具有良好的线性关系。线性范围为 20 至 200 μmol/L，检测限低至 6.6 μmol/L。从三名志愿者的实际汗液中检测 UA 得到了良好的结果。
本工作提供了两种用于 UA 检测的无酶比色检测系统。此外，还制备了一种简单、低成本、选择性好的柔性可穿戴微流控比色检测芯片，用于非侵入式、现场实时检测汗液 UA，在个人健康监测和即时检测方面具有很大的应用潜力。
