Chavda V R, Bhatt S B, Umaretiya V R, Gajera H P, Padhiyar S M, Kandoliya U K, Parakhia M V
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, India.
Sci Rep. 2025 Jan 3;15(1):632. doi: 10.1038/s41598-024-84840-z.
Endophytes are microorganisms residing in plant tissues without causing harm and their relevance in medicinal plants has grown due to their biomolecules used in pharmaceuticals. This study isolated two endophytic bacterial strains from the leaves of M. oleifera and P. betel collected from Junagadh Agricultural University. The isolates were characterized morphologically and physio-biochemically, confirming them as gram-positive or gram-negative rods and cocci. Identification using 16S rRNA gene sequencing identified isolates belonging to various genera, including Priestia aryabhattai and Kocuria rhizophila The SEM characterization of the five selected isolates revealed diverse morphological structures, including coccus and rod shapes, organized in various formations. Isolates varied in size, with N3 (Kocuria rhizophila) cocci and S5 (Priestia aryabhattai) rods. Metabolomic analysis using GC/MS and LC-MS revealed diverse metabolic profiles with key compounds like n-Hexadecanoic acid, Pyrrolo[1,2-a]pyrazine-1,4-dione, Dihydrocapsaicin, and β-Homoproline, highlighting the potential of these endophytic bacteria in agricultural applications due to their roles in membrane integrity, antioxidant properties, stress response, and antibacterial activity.
内生菌是存在于植物组织中且不会造成危害的微生物，由于它们的生物分子在制药领域的应用，其在药用植物中的相关性日益增加。本研究从朱纳加德农业大学采集的油橄榄和蒌叶叶片中分离出两株内生细菌菌株。对分离菌株进行了形态学和生理生化特征分析，确认它们为革兰氏阳性或革兰氏阴性杆菌和球菌。使用16S rRNA基因测序进行鉴定，确定分离菌株属于多个属，包括阿氏Priestia aryabhattai和嗜根考克氏菌Kocuria rhizophila。对五个选定分离菌株的扫描电子显微镜表征揭示了不同的形态结构，包括球菌和杆菌形状，以各种形式排列。分离菌株大小各异，N3（嗜根考克氏菌Kocuria rhizophila）为球菌，S5（阿氏Priestia aryabhattai）为杆菌。使用气相色谱/质谱联用仪（GC/MS）和液相色谱-质谱联用仪（LC-MS）进行的代谢组学分析揭示了不同的代谢谱，其中关键化合物有正十六烷酸、吡咯并[1,2-a]吡嗪-1,4-二酮、二氢辣椒素和β-高脯氨酸，突出了这些内生细菌在农业应用中的潜力，因为它们在膜完整性、抗氧化特性、应激反应和抗菌活性方面发挥作用。
