Sharma Mahima, Mallubhotla Sharada
Tissue Culture Laboratory, School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India.
Front Microbiol. 2022 May 13;13:879386. doi: 10.3389/fmicb.2022.879386. eCollection 2022.
Endophytic bacteria isolated from medicinal plants are crucial for the production of antimicrobial agents since they are capable of possessing bioactive compounds with diverse structures and activities. , a plant of medicinal importance native to the Jammu region of India, was selected for the isolation and characterization of culturable endophytic bacteria and evaluation of their antimicrobial activities. Standardized surface sterilization methods were employed to isolate thirty-three phenotypically distinguishable endophytic bacteria from the root, stem, and leaf parts of the plant. Shannon Wiener diversity index clearly divulged diverse endophytes in roots (0.85), stem (0.61), and leaf (0.54) tissues. Physio-biochemical features of the isolates differentiated the distinct variations in their carbohydrate utilization profile and NaCl tolerance. The endophytes produced an array of enzymes, namely, catalase, oxidase, amylase, cellulase, nitrate reductase, and lipase. The bacterial isolates belong to the genera , and predominantly. However, the antibiotic susceptibility pattern indicated that the isolates were mostly sensitive to erythromycin and streptomycin, while they were resistant to rifampicin, amoxicillin, and bacitracin. Interestingly, majority of the bacterial endophytes of showed antimicrobial activity against followed by . The 16S rRNA sequence of has been deposited in the NCBI GenBank database under accession number OM320575. The major compounds of the crude extract derived from endophytic OM320575, according to the metabolic profile examination by GC-MS, are dibutyl phthalate, eicosane, tetrapentacontane, heneicosane, and hexadecane, which possessed antibacterial activities. In conclusion, results indicated the potential of to host a plethora of bacterial endophytes that produce therapeutic bioactive metabolites.
从药用植物中分离出的内生细菌对于抗菌剂的生产至关重要，因为它们能够拥有结构和活性多样的生物活性化合物。印度查谟地区本土的一种具有药用价值的植物被选用于可培养内生细菌的分离和鉴定以及它们抗菌活性的评估。采用标准化的表面灭菌方法从该植物的根、茎和叶部分分离出33种表型可区分的内生细菌。香农维纳多样性指数清楚地揭示了根（0.85）、茎（0.61）和叶（0.54）组织中的多种内生菌。分离物的生理生化特征区分了它们在碳水化合物利用谱和耐NaCl能力方面的明显差异。内生菌产生了一系列酶，即过氧化氢酶、氧化酶、淀粉酶、纤维素酶、硝酸还原酶和脂肪酶。细菌分离物主要属于 属、 属和 属。然而，抗生素敏感性模式表明，分离物大多对红霉素和链霉素敏感，而对利福平、阿莫西林和杆菌肽耐药。有趣的是，该植物的大多数细菌内生菌对 表现出抗菌活性，其次是 。 的16S rRNA序列已保存在NCBI GenBank数据库中，登录号为OM320575。根据GC-MS代谢谱分析，内生菌OM320575粗提物的主要化合物是邻苯二甲酸二丁酯、二十烷、四十五烷、二十一烷和十六烷，它们具有抗菌活性。总之，结果表明该植物具有承载大量产生治疗性生物活性代谢物的细菌内生菌的潜力。
