Heart rate variability (HRV) is a key indicator of cardiac autonomic function, making reliable assessment crucial. To examine the test-retest stability of resting HRV in healthy individuals, fifty participants attended two lab sessions within a week, at the same time of day. After a 5-minute acclimatization period, electrocardiogram and respiration were recorded at rest. For validation, average heart rate and RMSSD were assessed over 15 minutes using a validated open-source toolbox. Test-retest agreement was evaluated using intra-class correlation (ICC), and coefficients of variation (CV). Mean heart rate showed high stability (ICC = 0.81, CV = 6%), while RMSSD had lower concordance (ICC = 0.75) and greater variation (CV = 30%). These findings indicate good test-retest agreement for standard HRV features. However, a wide range of methodologies exists for assessing various properties of heart rate dynamics. This database is intended to support other researchers in testing additional HRV metrics to evaluate their reliability in healthy individuals.