Mohamed Mostafa Kamel, Abdelrahman Mohamed Abdelfattah, Abdel-Razik Abdel-Razik Hashem, Elheeny Ahmad Abdel Hamid
Paediatric and Community Dentistry, Faculty of Dentistry, Minia University, Ard Shalaby, El Minia, 61519, Egypt.
Surgery, Anestheiology, and Radiology Department, Faculty of Veterinary Medicine, Minia University, El Minya, Egypt.
BMC Oral Health. 2025 Jan 3;25(1):12. doi: 10.1186/s12903-024-05297-0.
This study aimed to assess the histological and radiographic effects of sodium hexametaphosphate (SHMP) as a direct pulp capping (DPC) agent in immature permanent dog premolars.
A split-mouth design was employed with three healthy 4-month-old Mongrel dogs, each having 36 premolars. The premolars were randomly assigned to either SHMP or MTA. The specimens were stained with hematoxylin and eosin (H&E) and Masson's trichrome, and histologically examined three months after the animals were sacrificed. To assess root maturity, radiographic changes in root length (RL), root surface area (RSA), and apical foramen width (AFW) were measured at baseline and after 3 months. Quantitative data were analyzed using the paired-sample t-test, while the qualitative data based on Stanley's histological scoring system were tested using the Monte Carlo exact test. The level of significance was set at 5%.
Histological findings showed no significant differences between the two groups, except for the average thickness of the predentin and odontoblastic layers, which was significantly higher in the SHMP specimens (P < 0.0001). The frequencies of fully calcified dentin bridges and regularly arranged dentinal tubules were significantly higher in the SHMP specimens (P < 0.05). Both materials showed comparable radiographic measurements (P > 0.05), except for the change in RL, which was significantly longer in the SHMP group (P < 0.05).
There were no significant differences between SHMP and MTA in some respects. Histological evaluation showed that SHMP provided better bioinductive and biocompatible properties compared to MTA. Radiographically, both materials showed comparable root maturogenesis outcomes, except for the significant increase in RL in the SHMP group. SHMP may be a suitable alternative material for DPC in the treatment of immature permanent teeth.
本研究旨在评估六偏磷酸钠（SHMP）作为未成熟恒犬前磨牙直接盖髓剂（DPC）的组织学和影像学效果。
采用裂口设计，选取3只健康的4月龄杂种犬，每只犬有36颗前磨牙。将前磨牙随机分为SHMP组或MTA组。标本用苏木精和伊红（H&E）及马松三色染色，并在动物处死后3个月进行组织学检查。为评估牙根成熟度，在基线和3个月后测量牙根长度（RL）、牙根表面积（RSA）和根尖孔宽度（AFW）的影像学变化。定量数据采用配对样本t检验进行分析，而基于斯坦利组织学评分系统的定性数据采用蒙特卡罗精确检验进行检验。显著性水平设定为5%。
组织学结果显示，两组之间除前期牙本质和成牙本质细胞层的平均厚度外，无显著差异，SHMP标本中的该厚度显著更高（P < 0.0001）。SHMP标本中完全钙化的牙本质桥和规则排列的牙本质小管的频率显著更高（P < 0.05）。两种材料的影像学测量结果相当（P > 0.05），但RL的变化除外，SHMP组的RL显著更长（P < 0.05）。
SHMP和MTA在某些方面无显著差异。组织学评估表明，与MTA相比，SHMP具有更好的生物诱导和生物相容性。影像学上，两种材料的牙根成熟结果相当，但SHMP组的RL显著增加。SHMP可能是治疗未成熟恒牙DPC的合适替代材料。
