Albertsson M, Mercke C, Håkansson C H, von Mecklenburg C
Radiother Oncol. 1985 Jan;3(1):47-60. doi: 10.1016/s0167-8140(85)80008-6.
The trachea is often located in the treatment volume when irradiating malignant tumours in the thorax. In order to evaluate possible synergism between misonidazole and irradiation on this tissue, the following studies were made. Fifty rabbits were treated with daily injections of 100 mg misonidazole given i.p. on consecutive days from 1 to 10 days. Morphological investigations of the trachea were made with scanning electron microscopy (SEM), transmission electron microscopy (TEM) and light microscopy (LM). Physiological examinations were performed with recording of the ciliary beat frequency. The results were compared with those from a group of 100 rabbits given misonidazole in a similar manner and exposed to irradiation (2 Gy) 15-30 min after each injection. Ten rabbits were used as controls. The results are compared to the effect of fractionated irradiation alone with 2 Gy/day. Fractionated irradiation of the ciliary epithelium in the trachea of the rabbit has shown dose-dependent physiological and morphological effects. Misonidazole potentiates these effects of radiation with a more pronounced change of the ciliary beat frequency and an increased metabolic activity as could be visualized on TEM. The combination of drug and irradiation also induced a hyperplasia of the ciliary epithelium. Misonidazole itself had no effect on the ciliary beat frequency, but caused a hypoplasia of the ciliary epithelium.
在对胸部恶性肿瘤进行放疗时，气管常常位于治疗区域内。为了评估甲硝唑与放疗对该组织可能存在的协同作用，进行了以下研究。50只兔子连续10天每天经腹腔注射100毫克甲硝唑。采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）和光学显微镜（LM）对气管进行形态学研究。通过记录纤毛摆动频率进行生理学检查。将结果与另一组100只以类似方式给予甲硝唑并在每次注射后15 - 30分钟接受照射（2戈瑞）的兔子的结果进行比较。10只兔子用作对照。将结果与每天2戈瑞的分次照射单独产生的效果进行比较。对兔子气管纤毛上皮进行分次照射已显示出剂量依赖性的生理和形态学效应。甲硝唑增强了这些辐射效应，纤毛摆动频率变化更明显，并且如在透射电子显微镜下所见，代谢活性增加。药物与照射的联合还诱导了纤毛上皮的增生。甲硝唑本身对纤毛摆动频率没有影响，但导致纤毛上皮发育不全。
