Albertsson M, Hakansson C H, Mercke C
Scan Electron Microsc. 1986(Pt 3):1109-19.
The ciliated epithelium of the rabbit trachea was irradiated with daily fractions of 2 Gy to an accumulated dose of 20 Gy (TD: 2, 6, 10, 16, or 20 Gy). Fifteen to forty-five minutes before start of the first irradiation (treatment day 1), 5 mg cis-DDP was given by intraperitoneal injection to each rabbit. Examination was made 1 - 10 days after each fractionation schedule, when specimens were taken for investigations. Scanning electron microscope investigations showed a gradual development of ciliary damage from blebs on the cilia to swollen tips, broken and bent cilia and finally an epithelial lining with areas free from cilia with a surface covered with microvilli-like structures. SEM also showed cell loss, and remnants of dead cells on the surface together with detritus. By transmission electron microscope ciliary damage, cell death and cell loss of the ciliated cell layer as well as exfoliation of portions of goblet-like cells on the surface could be confirmed. The irradiated ciliated epithelium and the untreated control epithelium in each animal showed no difference in this respect. Thus no enhancement of the effects of radiation could be observed. The development of ultrastructural damage may be due to a cytotoxic effect of the drug on the ciliated epithelium. However, 19 days after the start of cis - DDP injection, a hyperplasia of the basal cell layer was observed, which indicates that the observed cytotoxicity of the drug is reversible and a normalisation occurs during the last days of observation in this study.
对兔气管的纤毛上皮进行每日分次照射，每次剂量为2 Gy，累积剂量达20 Gy（总剂量：2、6、10、16或20 Gy）。在首次照射开始前15至45分钟（治疗第1天），给每只兔腹腔注射5 mg顺铂。在每次分次照射方案后的1至10天进行检查，此时采集标本进行研究。扫描电子显微镜检查显示，纤毛损伤逐渐发展，从纤毛上的小泡到肿胀的尖端、断裂和弯曲的纤毛，最后是无纤毛区域的上皮内衬，其表面覆盖着微绒毛样结构。扫描电子显微镜还显示细胞丢失，表面有死亡细胞的残余物以及碎屑。通过透射电子显微镜可以证实纤毛损伤、纤毛细胞层的细胞死亡和细胞丢失以及表面部分杯状细胞的脱落。在这方面，每只动物中接受照射的纤毛上皮和未处理的对照上皮没有差异。因此，未观察到辐射效应的增强。超微结构损伤的发展可能是由于药物对纤毛上皮的细胞毒性作用。然而，在顺铂注射开始19天后，观察到基底细胞层增生，这表明在本研究的观察期最后几天，观察到的药物细胞毒性是可逆的，且出现了正常化。
