Lopes Ranieli Paiva, Máximo Vaz Millena Alves, Ferreira Fernanda Lopes, Sousa Grasiely Faria de, Magalhães Cintia Lopes de Brito, Vieira-Filho Sidney Augusto, Siqueira Ferreira Jaqueline Maria, Tótola Antônio Helvécio, Duarte Lucienir Pains, Carlos de Magalhães José
Laboratory of Virology and Cellular Technology, Department of Chemistry, Biotechnology, and Bioprocess Engineering, Universidade Federal de São João del-Rei, Ouro Branco, Brazil.
Department of Chemistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Drug Dev Ind Pharm. 2025 Feb;51(2):102-110. doi: 10.1080/03639045.2024.2449130. Epub 2025 Jan 17.
, a notable plant species, has garnered interest for its medicinal properties, including anti-inflammatory, antibacterial and antiviral effects. A vaccine for Chikungunia virus is still under evaluation and no specific antiviral drug has been licensed to date.
The work investigated antiviral activity of ethyl acetate (EAEF) and methanolic (EMF) extracts from leaves in mammalian cells exposed to (CHIKV).
The cytotoxicity, antiviral activity, selectivity index, effect on viral gene expression, virus production, and mechanisms of action were evaluated.
EAEF and EMF extracts showed anti-CHIKV effects at non-cytotoxic concentrations, with CC above 300 μg/mL, EC of 18 and 43 μg/mL respectively, and selectivity Index above 4. These concentrations drastically reduce viral yields and CHIKV gene expression and have shown activity both directly on viral particles and at different stages of the viral cycle.
EAEF and EMF showed robust antiviral activity against CHIKV, making them promising candidates for the development of anti-CHIKV drugs.
[植物名称]是一种著名的植物物种，因其药用特性而受到关注，包括抗炎、抗菌和抗病毒作用。基孔肯雅病毒疫苗仍在评估中，迄今为止尚无特定的抗病毒药物获得许可。
本研究调查了[植物名称]叶的乙酸乙酯提取物（EAEF）和甲醇提取物（EMF）对暴露于基孔肯雅病毒（CHIKV）的哺乳动物细胞的抗病毒活性。
评估了细胞毒性、抗病毒活性、选择性指数、对病毒基因表达的影响、病毒产生及作用机制。
EAEF和EMF提取物在无细胞毒性浓度下显示出抗CHIKV作用，CC50高于300μg/mL，EC50分别为18和43μg/mL，选择性指数高于4。这些浓度可大幅降低病毒产量和CHIKV基因表达，并且在直接作用于病毒颗粒以及病毒周期的不同阶段均显示出活性。
EAEF和EMF对CHIKV显示出强大的抗病毒活性，使其成为开发抗CHIKV药物的有前景的候选物。
