Santiago-Cruz José Angel, Posadas-Mondragón Araceli, Pérez-Juárez Angélica, Herrera-González Norma Estela, Chin-Chan José Miguel, Aguilar-González Joab Eli, Aguilar-Faisal José Leopoldo
Laboratorio de Medicina de Conservación de la Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico.
Laboratorio de Oncología Molecular de la Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico.
Viruses. 2024 Dec 17;16(12):1929. doi: 10.3390/v16121929.
Chikungunya virus (CHIKV) is classified as a pathogen with the potential to cause a pandemic. This situation becomes more alarming since no approved drug exists to combat the virus. The present research aims to demonstrate the anti-CHIKV activity of molecules present in the latex of . Therefore, a biodirected assay was carried out to find the molecules with anti-CHIKV activity. Extractions with hexane, dichloromethane, and methanol and subsequent purification by column chromatography were carried out to later evaluate cytotoxic activity by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and antiviral activity by plaque assay. Our findings show that unlike the others, methanolic extract has a low cytotoxic effect and a good anti-CHIKV effect (EC = 26.41 µg/mL), which increases when obtaining the purified active fraction (pAFeg1) (EC = 0.4835 µg/mL). Time-of-addition suggests that the possible mechanism of action of pAFeg1 could be inhibiting any of the non-structural proteins of CHIKV. In addition, both the cytotoxic and anti-CHIKV activity of pAFeg1 demonstrate selectivity since it killed cancer cells and could not inhibit DENV2.
基孔肯雅病毒（CHIKV）被归类为有可能引发大流行的病原体。由于目前尚无获批的抗该病毒药物，这种情况变得更加令人担忧。本研究旨在证明[植物名称]乳胶中存在的分子具有抗CHIKV活性。因此，开展了一项生物导向分析以寻找具有抗CHIKV活性的分子。分别用己烷、二氯甲烷和甲醇进行提取，随后通过柱色谱法进行纯化，之后通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑溴盐（MTT）法评估细胞毒性活性，并通过蚀斑试验评估抗病毒活性。我们的研究结果表明，与其他提取物不同，甲醇提取物具有较低的细胞毒性作用和良好的抗CHIKV效果（EC = 26.41 µg/mL），在获得纯化的活性组分（pAFeg1）时（EC = 0.4835 µg/mL），该效果会增强。加样时间表明，pAFeg1可能的作用机制是抑制CHIKV的任何一种非结构蛋白。此外，pAFeg1的细胞毒性和抗CHIKV活性均表现出选择性，因为它能杀死癌细胞且无法抑制登革热病毒2型（DENV2）。
