Xu Shasha, Zhai Yi, Wang Chen, Zhang Yang, Liu Xiaowei, Jiang Jianjun, Mi Yafei
Department of Cardiology, Taizhou Hospital of Zhejiang Province, affiliated to Wenzhou Medical University, Linhai, Zhejiang Province, China.
Laboratory of Cardiovascular Disease, Taizhou Hospital of Zhejiang Province, affiliated to Wenzhou Medical University, Linhai, Zhejiang Province, China.
Curr Med Chem. 2025;32(23):4816-4835. doi: 10.2174/0109298673346007241113072114.
This study was to explore the relationship between plasma exosomes and Acute myocardial infarction (AMI).
Acute myocardial infarction (AMI) is one of the most common cardiovascular complications. Recent studies have shown that exosomes play a crucial role in the development and progression of cardiovascular diseases. However, there is a lack of relevant research on the relationship between plasma exosomes and AMI.
This study was designed to explore the relationship between plasma exosomes and AMI.
This retrospective study collected the basic clinical data of patients with AMI (n = 10), stable angina pectoris (SAP, n = 10), and noncoronary heart disease (CON, n = 10) at the Department of Cardiovascular Medicine at Taizhou Hospital (Zhejiang, China, 2021.01 to 2021.12). Proteomics was used to systematically screen the differential proteins of plasma exosomes in patients with clinical AMI, SAP, and CON. Then, the results were further verified using parallel reaction monitoring (PRM).
Five of all the differentially expressed proteins (DEPs) were quantified by PRM. Compared with the CON group, heparin cofactor 2 (SERPIND1), mannan-binding lectin serine protease 1 (MASP1), ficolin-2 (FCN2), and α1-Microglobulin/bikunin precursor (AMBP) were upregulated in patients with AMI and SAP, with a higher expression in AMI than in SAP. Additionally, human leukocyte antigen (HLA-C) was downregulated in both exosomes and plasma.
The expression of four plasma exosome biomarkers in AMI and SAP patients was higher than that in noncoronary heart disease (NCHD) patients. HLA-C was downregulated in both exosomes and plasma, showing a potential to serve as a new candidate target for the detection and therapy of AMI.
本研究旨在探讨血浆外泌体与急性心肌梗死（AMI）之间的关系。
急性心肌梗死（AMI）是最常见的心血管并发症之一。最近的研究表明，外泌体在心血管疾病的发生和发展中起关键作用。然而，关于血浆外泌体与AMI之间的关系缺乏相关研究。
本研究旨在探讨血浆外泌体与AMI之间的关系。
这项回顾性研究收集了台州医院心血管内科（中国浙江，2021年1月至2021年12月）急性心肌梗死患者（n = 10）、稳定型心绞痛（SAP，n = 10）和非冠心病患者（CON，n = 10）的基本临床数据。采用蛋白质组学方法系统筛选临床AMI、SAP和CON患者血浆外泌体的差异蛋白。然后，使用平行反应监测（PRM）进一步验证结果。
所有差异表达蛋白（DEP）中的5种通过PRM进行了定量。与CON组相比，肝素辅因子2（SERPIND1）、甘露聚糖结合凝集素丝氨酸蛋白酶1（MASP1）、纤维胶凝蛋白-2（FCN2）和α1-微球蛋白/比昆宁前体（AMBP）在AMI和SAP患者中上调，且在AMI中的表达高于SAP。此外，人类白细胞抗原（HLA-C）在外泌体和血浆中均下调。
AMI和SAP患者中四种血浆外泌体生物标志物的表达高于非冠心病（NCHD）患者。HLA-C在外泌体和血浆中均下调，显示出作为AMI检测和治疗新候选靶点的潜力。
Zotero 插件