Ünübol Nihan, Caglayan Nese, Cebeci Sinan, Beşli Yeşim, Sancak Banu, Uyar Neval Yurttutan, Ahrabi Siavosh Salmanzadeh, Alebouyeh Masoud, Kocagöz Tanıl
Department of Medical Microbiology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey; Medical Laboratory Technician Program, Vocational School of Health Services, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
Department of Medical Biotechnology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
J Infect Public Health. 2025 Feb;18(2):102633. doi: 10.1016/j.jiph.2024.102633. Epub 2024 Dec 25.
Drug-resistant Group A beta-hemolytic streptococci remain significant infectious agents globally. This study investigated the major S. pyogenes strains responsible for infections in Türkiye and their susceptibility to beta-lactam and macrolide antibiotics.
We determined the minimum inhibitory concentration using the penicillin gradient test and performed emm typing and DNA fingerprinting via pulsed-field gel electrophoresis (PFGE) to analyze the clonal spread of 92 S. pyogenes strains isolated from two hospitals in Türkiye between 2020 and 2022.
Our findings revealed the predominant S. pyogenes strains causing infections in the population and provided insights into the epidemiological relatedness of these drug-resistant strains. This study also evaluated the correlation between emm typing and PFGE in tracking S. pyogenes epidemiology. In this study, the current resistance patterns of S. pyogenes strains in Türkiye identified erythromycin resistance in a few strains, but no resistance to penicillin was detected.
This study revealed that emm types 1, 12 and 89 as S. pyogenes strain genotypes were responsible for epidemic infections in Türkiye. PFGE genotyping and emm typing were found to provide better phylogenetic classification in the investigation of S. pyogenes epidemiology.
耐药物的A组β溶血性链球菌在全球范围内仍是重要的感染病原体。本研究调查了土耳其引起感染的主要化脓性链球菌菌株及其对β-内酰胺类和大环内酯类抗生素的敏感性。
我们使用青霉素梯度试验确定最低抑菌浓度，并通过脉冲场凝胶电泳（PFGE）进行emm分型和DNA指纹图谱分析，以分析2020年至2022年期间从土耳其两家医院分离出的92株化脓性链球菌菌株的克隆传播情况。
我们的研究结果揭示了在人群中引起感染的主要化脓性链球菌菌株，并提供了对这些耐药菌株流行病学相关性的见解。本研究还评估了emm分型和PFGE在追踪化脓性链球菌流行病学中的相关性。在本研究中，土耳其化脓性链球菌菌株目前的耐药模式显示少数菌株对红霉素耐药，但未检测到对青霉素的耐药性。
本研究表明，emm 1型、12型和89型作为化脓性链球菌菌株基因型是土耳其流行性感染的原因。发现PFGE基因分型和emm分型在化脓性链球菌流行病学调查中能提供更好的系统发育分类。
