Zhang Xuehui, Tang Lihua, Duan Linqing, Yang Ruihua, Liu Kangchengyin, Zhao Jing, Zhao Ye, Zhang Guozhong
National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Poult Sci. 2025 Feb;104(2):104751. doi: 10.1016/j.psj.2024.104751. Epub 2024 Dec 31.
The aim of this study was to investigate the molecular characteristics and pathogenicity of recently isolated ILTV strains from China, thereby augmenting our understanding of its prevalence. The complete genome sequences of seven ILTV strains obtained from China between 2015 and 2019 were determined by high-throughput sequencing. Phylogenetic analysis showed that six isolates (SD2015, GD2017, SYB2018, HB201812, HB201806, and TJ2019) were classified together with CEO vaccine strains, while only one isolates LN2018 belonged to the wild-type cluster. Recombination analysis revealed compelling evidence of probable recombination events existing in SD2015, HB201806, and LN2018. Compared to TJ2019 and LN2018, chickens infected with HB201806 exhibited more severe conjunctivitis symptoms and tracheal damage, as well as higher viral load and viral shedding in the trachea. These findings will provide valuable references for further understanding the epidemiological status of ILTV and developing effective control measures.
本研究的目的是调查最近从中国分离的传染性喉气管炎病毒（ILTV）毒株的分子特征和致病性，从而加深我们对其流行情况的了解。通过高通量测序确定了2015年至2019年间从中国获得的7株ILTV毒株的全基因组序列。系统发育分析表明，6株分离株（SD2015、GD2017、SYB2018、HB201812、HB201806和TJ2019）与CEO疫苗株归为一类，而只有1株分离株LN2018属于野生型簇。重组分析揭示了SD2015、HB201806和LN2018中可能存在重组事件的有力证据。与TJ2019和LN2018相比，感染HB201806的鸡表现出更严重的结膜炎症状和气管损伤，以及气管中更高的病毒载量和病毒脱落。这些发现将为进一步了解ILTV的流行病学状况和制定有效的控制措施提供有价值的参考。
