Liu Yiling, Chen Zixin, Chang Chaofeng, Lin Yifen, Zheng Guiyi, Zhang Feng
Engineering Technological Center of Fungus Active Substances of Fujian Province, College of Biological Sciences and Technology, Minnan Normal University, Zhangzhou, 363000, China.
Curr Microbiol. 2025 Jan 5;82(2):70. doi: 10.1007/s00284-024-04051-7.
Translation initiation, which involves numerous protein factors and coordinated control steps, represents the most complicated process during eukaryotic translation. However, the roles of eukaryotic translation initiation factor (eIF) in filamentous fungi are not well clarified. In this study, we investigated the function of eIF2Bα in Aspergillus oryzae, an industrially important filamentous fungus. The ΔeIF2Bα mutants showed slow colony growth and decreased conidia production, suggesting the critical roles of eIF2Bα in the growth and development of A. oryzae. In addition, the loss of eIF2Bα significantly impaired the ability to produce amylase and kojic acid, indicating the involvement of eIF2Bα in the amylase synthesis and secondary metabolite production. Interestingly, the elimination of eIF2Bα improved the tolerance of A. oryzae to diverse adverse stresses, including endoplasmic reticulum stress, oxidative stress, cell wall-perturbing stress, and cell membrane-damaging stress. Overall, our results indicate that eIF2Bα is a crucial regulator of growth, development, stress response, amylase production, and kojic acid synthesis in A. oryzae.
翻译起始涉及众多蛋白质因子和协同控制步骤，是真核生物翻译过程中最复杂的过程。然而，真核生物翻译起始因子（eIF）在丝状真菌中的作用尚未得到充分阐明。在本研究中，我们研究了工业上重要的丝状真菌米曲霉中eIF2Bα的功能。ΔeIF2Bα突变体显示出菌落生长缓慢和分生孢子产生减少，这表明eIF2Bα在米曲霉的生长和发育中起关键作用。此外，eIF2Bα的缺失显著损害了淀粉酶和 kojic 酸的产生能力，表明eIF2Bα参与了淀粉酶合成和次级代谢产物的产生。有趣的是，eIF2Bα的缺失提高了米曲霉对多种逆境胁迫的耐受性，包括内质网应激、氧化应激、细胞壁扰动应激和细胞膜损伤应激。总体而言，我们的结果表明eIF2Bα是米曲霉生长、发育、应激反应、淀粉酶产生和 kojic 酸合成的关键调节因子。
