Li Moli, Cheng Jie, Wang Huizhen, Shi Jiaoxia, Xun Xiaogang, Lu Wei, Wang Xubo, Hu Jingjie, Bao Zhenmin, Hu Xiaoli
MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution (Sanya 572024), Ocean University of China, China; National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution (Sanya 572024), Ocean University of China, China.
Ecotoxicol Environ Saf. 2025 Jan 1;289:117653. doi: 10.1016/j.ecoenv.2024.117653. Epub 2025 Jan 4.
Heat shock proteins (Hsps) are highly conserved molecular chaperones with essential roles against biotic and abiotic stressors. A large set of co-chaperons comprising J-domain proteins (DnaJs) regulate the ATPase cycle of Hsp70s with Hsp90s, together constituting a dynamic and functionally versatile network for protein folding/unfolding and regulation. Marine bivalves could accumulate and tolerate paralytic shellfish toxins (PSTs), the well-noted neurotoxins generated during harmful algal blooms. Here, 37 CfDnaJ and 35 PyDnaJ genes were systematically characterized in Zhikong scallop (Chlamys farreri) and Yesso scallop (Patinopecten yessoensis), the important aquaculture bivalve species in China. After exposure to different PST-producing dinoflagellates, Alexandrium minutum and Alexandrium catenella, diverse DnaJ regulations were presented in scallop hepatopancreas, accumulating incoming PSTs, and kidneys, transforming PSTs into higher toxic analogs. CfDnaJs' up-regulation in kidneys was similar with that in hepatopancreas, while their down-regulation was stronger in kidneys than in hepatopancreas, with CFA.38965.19.DNAJC30 being continuously down-regulated in both tissues of the two algae exposure. Moreover, PyDnaJs' up-regulation was only found in kidneys after A. catenella exposure, and PYE.10799.6.DNAJB1 was down-regulated in both tissues through the experiment. Together with the expression trends and correlation of DnaJ-Hsp70-Hsp90 genes, the organ-, toxin-, and species-dependent Hsp70B2 expressions were coordinately co-expressed with diverse DnaJ members, suggesting the functional diversity of scallop DnaJs with conserved Hsp70B2s in response to stress by PST-producing algae. Our results confirmed the regulated coordination of DnaJ-Hsp70B2 co-chaperons in scallops, and provided vital insights into the function and adaptation of scallop Hsps in response to PST stress.
热休克蛋白（Hsps）是高度保守的分子伴侣，在抵抗生物和非生物应激源方面发挥着重要作用。一大类包含J结构域蛋白（DnaJs）的共伴侣蛋白与Hsp90s一起调节Hsp70s的ATPase循环，共同构成一个动态且功能多样的蛋白质折叠/去折叠及调节网络。海洋双壳贝类能够积累并耐受麻痹性贝类毒素（PSTs），这是在有害藻华期间产生的著名神经毒素。在此，在中国重要的水产养殖双壳贝类物种栉孔扇贝（Chlamys farreri）和虾夷扇贝（Patinopecten yessoensis）中系统地鉴定了37个CfDnaJ和35个PyDnaJ基因。在暴露于不同产PST的甲藻微小亚历山大藻（Alexandrium minutum）和链状亚历山大藻（Alexandrium catenella）后，扇贝肝胰腺中呈现出不同的DnaJ调节情况，肝胰腺积累摄入的PSTs，而肾脏则将PSTs转化为毒性更强的类似物。CfDnaJs在肾脏中的上调与在肝胰腺中的情况相似，但其在肾脏中的下调比在肝胰腺中更强，在两种藻类暴露的两个组织中，CFA.38965.19.DNAJC30持续下调。此外，PyDnaJs的上调仅在暴露于链状亚历山大藻后的肾脏中被发现，并且在整个实验过程中，PYE.10799.6.DNAJB1在两个组织中均下调。结合DnaJ - Hsp70 - Hsp90基因的表达趋势和相关性，器官、毒素和物种依赖性的Hsp70B2表达与不同的DnaJ成员协同共表达，表明扇贝DnaJs与保守的Hsp70B2s在应对产PST藻类胁迫时功能具有多样性。我们的结果证实了扇贝中DnaJ - Hsp70B2共伴侣蛋白的调节协同作用，并为扇贝Hsps响应PST胁迫的功能和适应性提供了重要见解。
