Xiong Ningna, Liu Sili, Hu Wei, Liu Yamei, Ding Xiang, Xu Deming, Ling Yiqiang, Fang Manxin, Liu Ben, Zheng Lucheng, Zheng Wenya, Wu Bingyan
College of Life Science and Resources and Environment, Yichun University, Yichun, Jiangxi 336000, China.
College of Life Science and Resources and Environment, Yichun University, Yichun, Jiangxi 336000, China; Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, Yichun University, Yichun, Jiangxi 336000, China; Laboratory of Animal Pathogenic Microbiology, Yichun University, Yichun, Jiangxi 336000, China.
Anim Reprod Sci. 2025 Feb;273:107760. doi: 10.1016/j.anireprosci.2024.107760. Epub 2024 Dec 28.
Diquat (DQ) is a pro-oxidant that generates free radicals in cells through redox reactions, leading to the induction of oxidative stress. During the processes of growth and reproduction, poultry are particularly vulnerable to oxidative stress. Selenium yeast (SeY) serves as an organic selenium source characterized by high activity and low toxicity, imparting antioxidant effects. The objective of this study was to assess the protective effects of SeY against DQ-induced oxidative stress in rooster testicles.The results demonstrated that SeY pretreatment mitigated DQ-induced oxidative damage in the testes. This mitigation encompassed the alleviation of inhibited spermatogenesis, reduced spermatogenic cell abundance, and the alleviation of decreased expression of genes StAR, P450scc, and 3β-HSD which related to testosterone synthesis. Specifically, SeY pretreatment counteracted DQ-induced oxidative stress by activating the Nrf2/HO-1 antioxidant signaling pathway, enhancing the activity of antioxidant enzymes such as catalase (CAT) and total superoxide dismutase (T-SOD), and reducing the concentration of malondialdehyde (MDA). Furthermore, SeY pretreatment attenuated DQ-induced spermatogonia apoptosis by modulating the expression of apoptosis-related genes and proteins, including Bax, Bcl-2, Caspase3, and NF-κB. Additionally, SeY restored the proliferative capacity of spermatogenic cells by promoting the expression of the proliferation-related protein Ki67. The aforementioned findings signify that SeY effectively safeguards the testes against DQ-induced damage through mechanisms involving the reduction of oxidative stress, inhibition of apoptosis, promotion of proliferation, and enhancing the expression of testosterone synthesis related genes. This study lays a solid theoretical foundation for future research aimed at safeguarding the reproductive health of male poultry exposed to agricultural pesticides.
敌草快（DQ）是一种促氧化剂，可通过氧化还原反应在细胞中产生自由基，从而导致氧化应激的诱导。在生长和繁殖过程中，家禽特别容易受到氧化应激的影响。硒酵母（SeY）是一种有机硒源，具有高活性和低毒性的特点，具有抗氧化作用。本研究的目的是评估硒酵母对DQ诱导的公鸡睾丸氧化应激的保护作用。结果表明，硒酵母预处理减轻了DQ诱导的睾丸氧化损伤。这种减轻包括缓解精子发生抑制、减少生精细胞数量，以及缓解与睾酮合成相关的基因StAR、P450scc和3β-HSD表达的下降。具体而言，硒酵母预处理通过激活Nrf2/HO-1抗氧化信号通路、增强过氧化氢酶（CAT）和总超氧化物歧化酶（T-SOD）等抗氧化酶的活性以及降低丙二醛（MDA）浓度来对抗DQ诱导的氧化应激。此外，硒酵母预处理通过调节凋亡相关基因和蛋白质（包括Bax、Bcl-2、Caspase3和NF-κB）的表达来减轻DQ诱导的精原细胞凋亡。此外，硒酵母通过促进增殖相关蛋白Ki67的表达恢复了生精细胞的增殖能力。上述发现表明，硒酵母通过减少氧化应激、抑制凋亡、促进增殖以及增强睾酮合成相关基因的表达等机制有效地保护睾丸免受DQ诱导的损伤。本研究为未来旨在保护接触农用农药的雄性家禽生殖健康的研究奠定了坚实的理论基础。
