Ramdass Amanda C, Rampersad Sephra N
Biochemistry Research Lab (Rm216), Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago, West Indies.
DNA Res. 2024 Dec 27;32(1). doi: 10.1093/dnares/dsaf001.
Bacteria that are chronically exposed to high levels of pollutants demonstrate genomic and corresponding metabolic diversity that complement their strategies for adaptation to hydrocarbon-rich environments. Whole genome sequencing was carried out to infer functional traits of Serratia marcescens strain SMTT recovered from soil contaminated with crude oil. The genome size (Mb) was 5,013,981 with a total gene count of 4,842. Comparative analyses with carefully selected S. marcescens strains, 2 of which are associated with contaminated soil, show conservation of central metabolic pathways in addition to intra-specific genetic diversity and metabolic flexibility. Genome comparisons also indicated an enrichment of genes associated with multidrug resistance and efflux pumps for SMTT. The SMTT genome contained genes that enable the catabolism of aromatic compounds via the protocatechuate para-degradation pathway, in addition to meta-cleavage of catechol (meta-cleavage pathway II); gene enrichment for aromatic compound degradation was markedly higher for SMTT compared to the other S. marcescens strains analysed. Our data presents a valuable genetic inventory for future studies on strains of S. marcescens and provides insights into those genomic features of SMTT with industrial potential.
长期暴露于高浓度污染物中的细菌表现出基因组和相应的代谢多样性，这些多样性补充了它们适应富含碳氢化合物环境的策略。对从受原油污染的土壤中分离出的粘质沙雷氏菌菌株SMTT进行了全基因组测序，以推断其功能特性。该基因组大小（Mb）为5,013,981，总基因数为4,842个。与精心挑选的粘质沙雷氏菌菌株进行比较分析，其中2个与受污染土壤有关，结果表明除种内遗传多样性和代谢灵活性外，中心代谢途径具有保守性。基因组比较还表明，SMTT中与多药耐药性和外排泵相关的基因有所富集。SMTT基因组包含通过原儿茶酸对降解途径以及儿茶酚间位裂解（间位裂解途径II）实现芳香族化合物分解代谢的基因；与其他分析的粘质沙雷氏菌菌株相比，SMTT中芳香族化合物降解相关基因的富集程度明显更高。我们的数据为未来对粘质沙雷氏菌菌株的研究提供了宝贵的遗传资源，并深入了解了具有工业潜力的SMTT的那些基因组特征。
