Bidros Joy, Brock Kaitlyn, Gard Jaycie, Cooper Robin
Biology, University of Kentucky, Lexington, Kentucky, United States.
MicroPubl Biol. 2024 Dec 20;2024. doi: 10.17912/micropub.biology.001400. eCollection 2024.
The heart of larval serves as a model preparation in addressing cardiac function, as known genetic mutations can be mimicked to examine therapies. Pharmacological agents and function of proteins, like TRPA1, which affect ionic transport and ion concentrations can be investigated for their action on cardiac function in this model. To maintain function, the larval heart tube needs to remain viable; thus, a physiological saline is required. It was found that a reduced Mg level from the standard saline provides a more stable heartbeat, even in stressful conditions such as heat and increased expression levels of TRPA1 proteins.
幼虫心脏可作为研究心脏功能的模型，因为已知的基因突变可以被模拟以检验治疗方法。可以研究影响离子转运和离子浓度的药物制剂和蛋白质（如TRPA1）的功能，以考察它们在该模型中对心脏功能的作用。为维持心脏功能，幼虫心脏管需要保持存活；因此，需要一种生理盐水。研究发现，即使在高温和TRPA1蛋白表达水平增加等应激条件下，降低标准盐水中的镁含量也能提供更稳定的心跳。
