Sun Jiajin, Wang Zhonghao, Yan Xinyu, Zhao Yuqi, Tan Li, Miao Xuning, Zhao Rong, Huo Wenjie, Chen Lei, Li Qinghong, Liu Qiang, Wang Cong, Guo Gang
College of Animal Science, Shanxi Agricultural University, Taigu, China.
Front Vet Sci. 2024 Dec 20;11:1450241. doi: 10.3389/fvets.2024.1450241. eCollection 2024.
Aflatoxin B1 (AFB1) has been recognized as a serious health risk for ruminant animals. From a molecular perspective, indole-3-acid (IAA) possesses the potential to enhance the removal of AFB1 by rumen microbiota. Therefore, this study aims to investigate the impact of different concentrations of IAA on the removal of AFB1 by rumen microbiota using an technique. Experiment 1: interaction between AFB1 and rumen fermentation. Experiment 2: The study used a randomized design with five IAA levels (0, 15, 150, 1,500, and 7,500 mg/kg) to examine the effect of IAA on AFB1 removal and its impact on rumen fermentation. The results showed: (1) the content of AFB1 gradually decreased, removal rate of up to 75.73% after 24 h. AFB1 exposure altered the rumen fermentation pattern, with significantly decreased in the acetic acid/propionic acid ratio ( < 0.05). It significantly reduced the relative proportions of , , and ( < 0.05). (2) As the content of IAA increased, AFB1 exposure decreased. A total of 15 and 150 mg/kg IAA significantly mitigated the negative impact of AFB1 on key rumen bacteria ( and ), increased acetate levels and acetate/propionate ratio ( < 0.05). However, 1,500 mg/kg IAA lowered levels of propionate and isovalerate, adversely affected enzyme activities (pectinase, xylan and Carboxymethyl-cellulase) and relative proportions of microbiota (, and ). In conclusion, IAA significantly removed AFB1, and in the range of 150 mg/kg of IAA reduced the negative effects of AFB1 on fermentation characteristics and fermentation end-products.
黄曲霉毒素B1（AFB1）已被公认为反刍动物面临的严重健康风险。从分子角度来看，吲哚 - 3 - 酸（IAA）具有增强瘤胃微生物群对AFB1清除能力的潜力。因此，本研究旨在使用一种技术研究不同浓度的IAA对瘤胃微生物群去除AFB1的影响。实验1：AFB1与瘤胃发酵之间的相互作用。实验2：该研究采用随机设计，设置五个IAA水平（0、15、150、1500和7500 mg/kg），以研究IAA对AFB1去除的影响及其对瘤胃发酵的影响。结果表明：（1）AFB1含量逐渐降低，24小时后去除率高达75.73%。AFB1暴露改变了瘤胃发酵模式，乙酸/丙酸比值显著降低（P<0.05）。它显著降低了[此处原文缺失具体物质]、[此处原文缺失具体物质]和[此处原文缺失具体物质]的相对比例（P<0.05）。（2）随着IAA含量增加，AFB1暴露量降低。15和150 mg/kg的IAA显著减轻了AFB1对瘤胃关键细菌（[此处原文缺失具体细菌名称]和[此处原文缺失具体细菌名称]）的负面影响，提高了乙酸水平和乙酸/丙酸比值（P<0.05）。然而，1500 mg/kg的IAA降低了丙酸和异戊酸水平，对酶活性（果胶酶、木聚糖酶和羧甲基纤维素酶）以及微生物群的相对比例（[此处原文缺失具体微生物名称]、[此处原文缺失具体微生物名称]和[此处原文缺失具体微生物名称]）产生了不利影响。总之，IAA显著去除了AFB1，在150 mg/kg的IAA范围内降低了AFB1对[此处原文缺失具体发酵相关内容]发酵特性和发酵终产物的负面影响。
