Wang Feibing, Niu Haofei, Xin Dongqing, Long Yi, Wang Guangpeng, Liu Zongmei, Li Gang, Zhang Fan, Qi Mingyang, Ye Yuxiu, Wang Zunxin, Pei Baolei, Hu Laibao, Yuan Caiyong, Chen Xinhong
School of Life Sciences and Food Engineering, Huaiyin Institute of Technology, Huai'an, China.
Huaiyin Institute of Agricultural Sciences of Xuhuai Region, Huai'an, China.
Front Plant Sci. 2021 Nov 18;12:738660. doi: 10.3389/fpls.2021.738660. eCollection 2021.
Auxin/indoleacetic acid (Aux/IAA) proteins play an important regulatory role in the developmental process of plants and their responses to stresses. A previous study has shown that constitutive expression of , an Aux/IAA transcription factor gene of rice improved salt and osmotic tolerance in transgenic plants. However, little work is known about the regulatory functions of the gene in regulating the abiotic stress tolerance of rice. In this study, the gene was introduced into the rice cultivar, Zhonghua 11 and the overexpression in rice plants exhibited significantly enhanced salt and drought tolerance compared to the wild type (WT). Moreover, overexpression of in rice increased endogenous levels of abscisic acid (ABA) and the overexpression of in rice plants showed hypersensitivity to exogenous ABA treatment at both the germination and postgermination stages compared to WT. Overexpression of upregulated the genes involved in ABA biosynthesis and signaling pathways, proline biosynthesis pathway, and reactive oxygen species (ROS)-scavenging system in the overexpression of in rice plants under salt and drought stresses. Proline content, superoxide dismutase (SOD), and peroxidase (POD) activities were significantly increased, whereas malonaldehyde (MDA), hydrogen peroxide (HO), and superoxide anion radical (O ) content were significantly decreased in the transgenic plants under salt and drought stresses. Taken together, we suggest that plays a positive role in drought and salt tolerance by regulating stress-induced ABA signaling. The gene has a potential application in genetically modified crops with enhanced tolerance to abiotic stresses.
生长素/吲哚乙酸（Aux/IAA）蛋白在植物发育过程及其对胁迫的响应中发挥着重要的调节作用。先前的一项研究表明，水稻Aux/IAA转录因子基因的组成型表达提高了转基因植物的耐盐性和渗透耐受性。然而，关于该基因在调节水稻非生物胁迫耐受性方面的调控功能，人们所知甚少。在本研究中，将该基因导入水稻品种中花11，与野生型（WT）相比，该基因在水稻植株中的过表达表现出显著增强的耐盐性和耐旱性。此外，水稻中该基因的过表达增加了内源脱落酸（ABA）水平，与WT相比，该基因在水稻植株中的过表达在萌发期和萌发后期对外源ABA处理均表现出超敏反应。在盐胁迫和干旱胁迫下，该基因的过表达上调了水稻植株中参与ABA生物合成和信号通路、脯氨酸生物合成途径以及活性氧（ROS）清除系统的基因。在盐胁迫和干旱胁迫下，转基因植物中的脯氨酸含量、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性显著增加，而丙二醛（MDA）、过氧化氢（HO）和超氧阴离子自由基（O）含量显著降低。综上所述，我们认为该基因通过调节胁迫诱导的ABA信号在干旱和耐盐性中发挥积极作用。该基因在对非生物胁迫耐受性增强的转基因作物中具有潜在应用价值。
