来自中国的产超毒力KPC-2且耐多药的ST11-KL25的基因组特征分析
Genomic characterization of ST11-KL25 hypervirulent KPC-2-producing multidrug-resistant from China.
作者信息
Kang Yuting, Xu Chao, Ma Wanting, Li Qiujie, Jia Wei, Wang Pengtao
机构信息
Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China.
First Clinical Medical College, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
出版信息
iScience. 2024 Nov 27;27(12):111471. doi: 10.1016/j.isci.2024.111471. eCollection 2024 Dec 20.
The global prevalence of ST11 hypervirulent carbapenem-resistant (hv-CRKP) isolates has been increasingly documented, yet genomic characterization of this clone remains insufficiently explored. Here, we report a clinical ST11-KL25 hv-CRKP strain (KP156) that exhibited resistance to multiple antibiotics and demonstrated hypervirulence in a mouse infection model. Whole-genome sequencing revealed that KP156 harbored one virulence plasmid (pKP156-Vir) and two resistance plasmids (pKP156-KPC and pKP156-tetA). The pKP156-Vir contains several virulence factors, including and , which are critical contributors to its hypervirulence. The and genes, located within the Tn transposon of pKP156-KPC, along with a multidrug-resistant (MDR) region containing multiple transposons and conjugative elements in pKP156-tetA, are associated with the transfer of resistance genes. Phylogenetic analysis indicates that KP156 shares high homology with other ST11 hv-CRKPs, suggesting potential transmission of this clone. Our study informs the development of genomic surveillance and control strategies for this strain.
全球范围内，ST11型高毒力耐碳青霉烯类肺炎克雷伯菌（hv-CRKP）分离株的流行情况已有越来越多的文献记载，但对该克隆株的基因组特征仍缺乏充分研究。在此，我们报告了一株临床ST11-KL25 hv-CRKP菌株（KP156），它对多种抗生素具有耐药性，并在小鼠感染模型中表现出高毒力。全基因组测序显示，KP156携带一个毒力质粒（pKP156-Vir）和两个耐药质粒（pKP156-KPC和pKP156-tetA）。pKP156-Vir包含几个毒力因子，包括 和 ，它们是其高毒力的关键因素。位于pKP156-KPC的Tn转座子内的 和 基因，以及pKP156-tetA中包含多个转座子和接合元件的多药耐药（MDR）区域，与耐药基因的转移有关。系统发育分析表明，KP156与其他ST11 hv-CRKPs具有高度同源性，提示该克隆株可能存在传播。我们的研究为该菌株的基因组监测和控制策略的制定提供了依据。
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