大鼠肝脏中第三种金属硫蛋白（金属硫蛋白-II'）的诱导与检测
Induction and detection of a third isometallothionein (metallothionein-II') in rat liver.
作者信息
Suzuki K T, Uehara H, Sunaga H, Shimojo N
出版信息
Toxicol Lett. 1985 Jan;24(1):15-20. doi: 10.1016/0378-4274(85)90133-x.
PMID:3975925
Abstract
A third isometallothionein was shown to be present during metallothionein synthesis, particularly following induction by zinc (Zn). It appeared to be closely related to metallothionein-II and the relative amount decreased with time, with only a minor peak after repeated cadmium (Cd) loading. On-line column switching by high-performance liquid chromatography (HPLC)-atomic absorption spectrophotometry (AAS) is effective in analysing and characterizing isometallothioneins.
摘要
在金属硫蛋白合成过程中，尤其是在锌（Zn）诱导后，发现了第三种异金属硫蛋白。它似乎与金属硫蛋白-II密切相关，其相对含量随时间减少，在反复镉（Cd）加载后只有一个小峰值。通过高效液相色谱（HPLC）-原子吸收光谱法（AAS）进行在线柱切换，对于分析和表征异金属硫蛋白是有效的。
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