Rapp R
Stain Technol. 1985 Jan;60(1):39-43. doi: 10.3109/10520298509113889.
Calcified human permanent and primary teeth are often split to obtain pulp tissue for histochemical studies as well as unaltered dentin and surfaces for scanning electron microscopy. Various procedures have been used to cleave teeth, with different degrees of consistency. For rapid and fairly consistent splitting, a vise to the jaws of which triangular metal files have been welded has been found useful. The apices of the files were ground by an electric drill to the shape of typical teeth. Teeth to be split were grooved on their opposing external surfaces and were then cracked open between the file blades upon application of pressure by the vise. Teeth usually split lengthwise, exposing the entire pulp organ in one section and an empty pulp chamber-root canal in the other. This facilitated rapid penetration of fixative into pulp, and easier removal of pulp tissue in toto, as well as providing fresh enamel and dentin surfaces suitable for scanning electron microscopy.
钙化的人类恒牙和乳牙常常被劈开，以获取用于组织化学研究的牙髓组织，以及用于扫描电子显微镜检查的未改变的牙本质和表面。人们使用了各种方法来劈开牙齿，其一致性程度各不相同。为了快速且相当一致地劈开牙齿，人们发现一种在钳口上焊接了三角形金属锉的虎钳很有用。锉刀的尖端用电钻磨成典型牙齿的形状。要劈开的牙齿在其相对的外表面开槽，然后在虎钳施加压力时在锉刀刀片之间裂开。牙齿通常纵向劈开，在一个断面中露出整个牙髓器官，在另一个断面中露出空的髓腔 - 根管。这有助于固定剂快速渗透到牙髓中，更易于完整地取出牙髓组织，还能提供适合扫描电子显微镜检查的新鲜釉质和牙本质表面。
