Fauziah Ardina Nur, Kim Min Jeong, So Bo Ram, Son Joe Eun, Jung Sung Keun
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea.
Research Institute of Tailored Food Technology, Kyungpook National University, Daegu 41566, Korea.
Prev Nutr Food Sci. 2024 Dec 31;29(4):504-511. doi: 10.3746/pnf.2024.29.4.504.
L. cultivar Dangjo (DJ), developed as a novel crop, possesses potential health benefits, such as reducing blood glucose levels. DJ contains flavonoid glycosides, bioactive compounds that have been found in various plants and have promising pharmacological effects. A representative flavonol glycoside is quercitrin, a standard compound. Notwithstanding, its adoption by the food industry, it is necessary to standardize the quantification of quercitrin in DJ. Thus, an analytical method needs to be developed and validated to quantify quercitrin accurately. In this study, we established a high-performance liquid chromatography (HPLC) method for quantifying quercitrin in DJ extracts (DJEs); then, we validated the method, to ensure its accuracy and reliability. Our results demonstrated that the HPLC method effectively detecteds quercitrin in DJE samples, consistently reporting retention times and peak spectra similar to those in the standard solutions. The linearity assessment revealed a linear response within the concentration range of 2.5 to 15.0 μg/mL, which was supported by strong correlation coefficients (R>0.9997). Accuracy assessment via recovery studies produced satisfactory results (89.02%－99.30%), with a relative standard deviation (RSD) within acceptable limits (0.50%－5.95%). Precision analysis confirmed the repeatability and reproducibility of the method, with RSD values within the Association of Official Agricultural Chemists (AOAC) standard criteria (≤8%). Overall, our study provides a validated HPLC method for quercitrin quantification in DJEs, facilitating its standardization and ensuring the accuracy of the analysis. This method is potentially valuable for quality control and further research on the health-promoting properties of DJ.
作为一种新型作物培育的 Dangjo（DJ）品种具有潜在的健康益处，比如降低血糖水平。DJ 含有黄酮糖苷，这种生物活性化合物在多种植物中都有发现，且具有良好的药理作用。一种具有代表性的黄酮醇糖苷是槲皮苷，它是一种标准化合物。尽管如此，在食品工业采用它的过程中，有必要对 DJ 中槲皮苷的定量进行标准化。因此，需要开发并验证一种分析方法来准确地定量槲皮苷。在本研究中，我们建立了一种高效液相色谱（HPLC）方法来定量 DJ 提取物（DJEs）中的槲皮苷；然后，我们对该方法进行了验证，以确保其准确性和可靠性。我们的结果表明，HPLC 方法有效地检测出了 DJE 样品中的槲皮苷，其保留时间和峰谱始终与标准溶液中的相似。线性评估显示在 2.5 至 15.0 μg/mL 的浓度范围内呈线性响应，这得到了强相关系数（R>0.9997）的支持。通过回收率研究进行的准确性评估产生了令人满意的结果（89.02%－99.30%），相对标准偏差（RSD）在可接受范围内（0.50%－5.95%）。精密度分析证实了该方法的可重复性和再现性，RSD 值在官方农业化学家协会（AOAC）标准范围内（≤8%）。总体而言，我们的研究提供了一种经过验证的 HPLC 方法用于定量 DJEs 中的槲皮苷，有助于其标准化并确保分析的准确性。该方法对于 DJ 的质量控制和促进健康特性的进一步研究可能具有重要价值。
