Dawane Jayshree, Seok Sanghee, Dhande Priti, Langade Deepak, Han Hwana, Kim Sang-Back, Ju Jae-Yeong
Bharati Vidyapeeth Deemed University Medical College, Pune 411043, India.
Department of Global Planning, Kolmar BNH, Seoul 06800, Korea.
Prev Nutr Food Sci. 2024 Dec 31;29(4):414-421. doi: 10.3746/pnf.2024.29.4.414.
Ashwagandha () is a popular herb in Ayurveda, the traditional medicine system in India. It is known to exert stress-mitigating properties and has been extensively studied for its safety and efficacy in various disorders. This study assessed the effects of Ashwagandha root extract (ARE) on stress in rats. The anxiolytic and antidepressant effects of ARE were assessed using the elevated plus maze test, sucrose preference test, and forced swim test. The rats were divided into the following groups: control group (no disease), disease control group (no treatment), standardized ARE group (test; ARE administered in doses of 27, 54, and 108 mg/kg body weight), and fluoxetine group (active control). Biochemical parameters in the serum [monoamine oxidase (MAO)-A, MAO-B, serotonin, cortisol, adrenocorticotropic hormone (ACTH), corticotropin-releasing hormone (CRH), interleukin (IL)-6, tumor necrosis factor (TNF)-α, and brain-derived neurotrophic factor (BDNF)] and brain tissue (serotonin) were estimated at the end of 36 days to understand the potential mechanism behind the anxiolytic and antidepressant effects of ARE. The behavior test results indicated significant improvement in anxiety and depression-like behavior with ARE treatment in a rat model exposed to a validated protocol of chronic variable stress. The results of biochemical analyses revealed a significant increase in serotonin and BDNF levels and a decrease in CRH, ACTH, and cortisol levels. The inflammatory markers IL-6 and TNF-α were also significantly reduced with ARE treatment. ARE demonstrated notable effects on anxiety and depression markers in rats, indicating its potential as a prophylactic and therapeutic agent.
印度人参（）是印度传统医学阿育吠陀中一种广为人知的草药。它具有减轻压力的特性，并且已经针对其在各种疾病中的安全性和有效性进行了广泛研究。本研究评估了印度人参根提取物（ARE）对大鼠应激的影响。使用高架十字迷宫试验、蔗糖偏好试验和强迫游泳试验评估了ARE的抗焦虑和抗抑郁作用。将大鼠分为以下几组：对照组（无疾病）、疾病对照组（未治疗）、标准化ARE组（试验组；以27、54和108mg/kg体重的剂量给予ARE）和氟西汀组（阳性对照组）。在36天结束时评估血清中的生化参数[单胺氧化酶（MAO）-A、MAO-B、血清素、皮质醇、促肾上腺皮质激素（ACTH）、促肾上腺皮质激素释放激素（CRH）、白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α和脑源性神经营养因子（BDNF）]以及脑组织（血清素），以了解ARE抗焦虑和抗抑郁作用背后的潜在机制。行为测试结果表明，在暴露于经过验证的慢性可变应激方案的大鼠模型中，ARE治疗可显著改善焦虑和抑郁样行为。生化分析结果显示血清素和BDNF水平显著升高，CRH、ACTH和皮质醇水平降低。ARE治疗还显著降低了炎症标志物IL-6和TNF-α。ARE对大鼠的焦虑和抑郁标志物有显著影响，表明其作为预防和治疗药物的潜力。
