Research Group of Innovative Special Food, Korea Food Research Institute, 62, Anyangpangyo-ro, Bundang-gu, Seongnam, 13539, Republic of Korea.
Department of Food Biotechnology, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.
Inflammopharmacology. 2018 Feb;26(1):207-215. doi: 10.1007/s10787-017-0389-1. Epub 2017 Aug 28.
The root of Withania somnifera, commonly known as ashwagandha, is a traditional herb in the Indian Ayurvedic system of medicine and is used as a tonic. Here, we investigated whether W. somnifera root extract exhibits analgesic effects in plantar incision (PI) and spared nerve injury (SNI) rat models. Mechanical withdrawal threshold (MWT) was measured by von Frey filaments, and pain-related behavior was determined after operation by ultrasonic vocalization (USV) measurements. Indeed, we examined interferon-γ (IFN-γ) and interleukin-10 (IL-10) levels in the isolated dorsal root ganglia (DRG) following SNI in rats using an ELISA cytokine assay. MWT significantly increased 6 and 24 h after PI in rats receiving W. somnifera root extracts (100 and 300 mg/kg). Furthermore, the number of 22-27-kHz USV, which are a distress response, was significantly reduced at 6 and 24 h after PI in W. somnifera-treated rats (100 and 300 mg/kg). SNI-induced hyperalgesia and cytokine levels were significantly alleviated after treating with W. somnifera root extracts (100 and 300 mg/kg) for 15 continuous days. The main active compound, withaferin A, from the W. somnifera root extract has shown the CC chemokine family Receptor 2 (CCR2) antagonistic effects on monocyte chemoattractant protein-1 (MCP-1)-induced Ca response in CCR2 stable cell line. These results indicate that W. somnifera root extract has a potential analgesic effect in rat models for both postoperative and neuropathic pain and shows potential as a drug or supplement for the treatment of pain.
印度草医学体系中，睡茄的根部，通常被称为 Ashwagandha，被用作滋补品。在这里，我们研究了睡茄根提取物是否在足底切口 (PI) 和 spared 神经损伤 (SNI) 大鼠模型中表现出镇痛作用。通过 Von Frey 纤维测量机械退缩阈值 (MWT)，并在手术后通过超声发声 (USV) 测量确定与疼痛相关的行为。实际上，我们使用 ELISA 细胞因子测定法检查了 SNI 后大鼠分离的背根神经节 (DRG) 中干扰素-γ (IFN-γ) 和白细胞介素-10 (IL-10) 的水平。MWT 在接受睡茄根提取物 (100 和 300mg/kg) 的大鼠 PI 后 6 和 24 小时显着增加。此外，在 PI 后 6 和 24 小时，在接受睡茄治疗的大鼠 (100 和 300mg/kg) 中，22-27kHz USV 的数量显着减少，这是一种痛苦反应。SNI 诱导的痛觉过敏和细胞因子水平在连续 15 天用睡茄根提取物 (100 和 300mg/kg) 治疗后显着减轻。来自睡茄根提取物的主要活性化合物，Withaferin A，已显示对单核细胞趋化蛋白-1 (MCP-1) 诱导的 CCR2 稳定细胞系 Ca 反应具有趋化因子家族受体 2 (CCR2) 拮抗作用。这些结果表明，睡茄根提取物在大鼠术后和神经病理性疼痛模型中具有潜在的镇痛作用，并显示出作为治疗疼痛的药物或补充剂的潜力。
