Rese Morten, van Erven Gijs, Veersma Romy J, Alfredsen Gry, Eijsink Vincent G H, Kabel Mirjam A, Tuveng Tina R
Faculty of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, Ås 1433, Norway.
Wageningen Food and Biobased Research, Bornse Weilanden 9, Wageningen 6708 WG, The Netherlands.
Biomacromolecules. 2025 Feb 10;26(2):1063-1074. doi: 10.1021/acs.biomac.4c01403. Epub 2025 Jan 6.
Wood-degrading brown-rot fungi primarily target carbohydrates, leaving the lignin modified and potentially valuable for valorization. Here, we report a comprehensive comparison of how degrades hardwood and softwood, which have fundamentally different lignin structures. By harnessing the latest advancements in analytical methodologies, we show that removes more lignin from wood (up to 36%) than previously reported. The brown-rot decayed lignin appeared substantially C-oxidized, -demethylated, with a reduction in interunit linkages, leading to formation of substructures indicative of C-C, β-, and -4 cleavage. Our work shows that the conversion of hardwood and softwood lignin results in similar modifications, despite the structural differences. Furthermore, lignin modification by enhances the antioxidant capacity of the lignin and generates an extractable lower molecular weight fraction. These findings improve our understanding of lignin conversion by brown-rot fungi and highlight their biotechnological potential for the development of lignin-based products.
降解木材的褐腐真菌主要作用于碳水化合物，使木质素发生改性，从而可能具有增值价值。在此，我们报告了对一种真菌如何降解具有根本不同木质素结构的阔叶材和针叶材的全面比较。通过利用分析方法的最新进展，我们发现该真菌从木材中去除的木质素比之前报道的更多（高达36%）。褐腐腐朽的木质素呈现出显著的碳氧化、去甲基化，单元间连接减少，导致形成了表明碳 - 碳、β - 和β - 4断裂的亚结构。我们的研究表明，尽管结构存在差异，但该真菌对阔叶材和针叶材木质素的转化会产生相似的改性。此外，该真菌对木质素的改性增强了木质素的抗氧化能力，并产生了可提取的低分子量部分。这些发现增进了我们对褐腐真菌转化木质素的理解，并突出了它们在开发木质素基产品方面的生物技术潜力。
