Rumpf Jessica, Burger René, Schulze Margit
Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, Germany; Agricultural Faculty, University of Bonn, Meckenheimer Allee 174, D-53115 Bonn, Germany.
Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, Germany.
Int J Biol Macromol. 2023 Apr 1;233:123470. doi: 10.1016/j.ijbiomac.2023.123470. Epub 2023 Feb 2.
This research studies in detail four different assays, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), to determine the antioxidant capacity of standard substances as well as 50 organosolv lignins, and two kraft lignins. The coefficient of variation was determined for each method and was lowest for ABTS and highest for DPPH. The best correlation was found for FRAP and FC, which both rely on a single electron transfer mechanism. A good correlation between ABTS, FRAP and FC, respectively, could be observed, even though ABTS relies on a more complex reaction mechanism. The DPPH assay merely correlates with the others, implying that it reflects different antioxidative attributes due to a different reaction mechanism. Lignins obtained from paulownia and silphium have been investigated for the first time regarding their antioxidant capacity. Paulownia lignin is in the same range as beech wood lignin, while silphium lignin resembles wheat straw lignin. Miscanthus lignin is an exception from the grass lignins and possesses a significantly higher antioxidant capacity. All lignins possess a good antioxidant capacity and thus are promising candidates for various applications, e. g. as additives in food packaging or for biomedical purposes.
本研究详细考察了四种不同的分析方法，即DPPH（2,2-二苯基-1-苦基肼）、ABTS（2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸））、FRAP（铁离子还原抗氧化能力）和FC（福林-酚法），以测定标准物质以及50种有机溶剂木质素和两种硫酸盐木质素的抗氧化能力。测定了每种方法的变异系数，其中ABTS法的变异系数最低，DPPH法的变异系数最高。发现FRAP和FC的相关性最好，这两种方法都依赖于单电子转移机制。尽管ABTS依赖于更复杂的反应机制，但分别观察到ABTS、FRAP和FC之间有良好的相关性。DPPH分析仅与其他方法有一定相关性，这意味着由于反应机制不同，它反映了不同的抗氧化特性。首次研究了泡桐和水飞蓟中木质素的抗氧化能力。泡桐木质素与山毛榉木质素处于同一水平，而水飞蓟木质素与小麦秸秆木质素相似。芒草木质素是禾本科木质素中的一个例外，具有显著更高的抗氧化能力。所有木质素都具有良好的抗氧化能力，因此有望用于各种应用，例如作为食品包装中的添加剂或用于生物医学目的。
