Baindara Piyush, Roy Dinata, Boosani Chandra S, Mandal Santi M, Green Jonathan A
Animal Sciences Research Center, Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA.
Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Kolkata, India.
Appl Environ Microbiol. 2025 Feb 19;91(2):e0170224. doi: 10.1128/aem.01702-24. Epub 2025 Jan 6.
Antimicrobial peptides (AMPs) have emerged as potential alternatives to conventional antibiotics due to their novelty and multiple mechanisms of action. Because they are peptides, AMPs are amenable to bioengineering and suitable for cloning and expression at large production scales. However, the efficient delivery of AMPs is an unaddressed issue, particularly due to their large size, possible toxicities, and the development of adverse immune responses. Here, we have reviewed the possibilities of adeno-associated virus (AAV)-based localized gene delivery of AMPs for the treatment of infectious diseases with a special focus on respiratory infections. By discussing the gene delivery mechanism of AAV and the accompanying technical and therapeutic challenges with AMPs, we describe a foundation that emphasizes the use of viral vector-based gene delivery of AMPs for disease treatment.
抗菌肽（AMPs）因其新颖性和多种作用机制，已成为传统抗生素的潜在替代品。由于它们是肽，抗菌肽适合进行生物工程改造，并且适合大规模克隆和表达。然而，抗菌肽的有效递送是一个尚未解决的问题，特别是由于它们的分子量大、可能存在的毒性以及不良免疫反应的发生。在这里，我们综述了基于腺相关病毒（AAV）的抗菌肽局部基因递送用于治疗传染病的可能性，特别关注呼吸道感染。通过讨论腺相关病毒的基因递送机制以及与抗菌肽相关的技术和治疗挑战，我们描述了一个强调使用基于病毒载体的抗菌肽基因递送来治疗疾病的基础。
