Liu Tianpeng, Dong Kongjun, He Jihong, Wang Mei, Ren Ruiyu, Zhang Lei, Li Yawei, Liu Minxuan, Yang Tianyu
Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China.
Key Laboratory of Crop Gene Resources and Germplasm Innovation in Northwest Cold and Arid Regions (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Lanzhou, 730070, China.
Theor Appl Genet. 2025 Jan 6;138(1):17. doi: 10.1007/s00122-024-04773-z.
A major locus SC9.1 was identified and finely mapped into a 92.68 Kb region, and longmi004412 was identified as the casual gene regulating brown seed color in broomcorn millet. Broomcorn millet is a cereal crop with abundant genetic variations in morphology, agronomy, and yield-related traits. The diversity of seed color is among the most distinctive morphological characteristics. However, genetic determinants governing seed coloration have rarely been reported. Here, the F and F populations from a cross between Longmi12 and Zhang778 were employed to elucidate the genetic basis of seed color. Statistical analysis conducted on the seed color in F, F and F progeny conclusively demonstrated that brown seed color was controlled by a single dominant locus in broomcorn millet. The genetic control locus, SC9.1, was preliminarily located on chromosome 9 in the 32,175,878-44,281,406 bp region through bulked segregant analysis sequencing (BSA-seq). Furthermore, SC9.1 was narrowed down to a 92.68 kb interval harboring 11 genes using fine mapping with 260 recessive individual genotypes. Combined with gene structural variation, the transcriptome profile, and functional comparison, longmi004412 was identified as the causal gene resulting in brown seed color formation in broomcorn millet. In addition, haplotype analysis of the longmi004412 gene in 516 accessions was performed to clarify the types for broomcorn millet seed color. These findings lay the foundation for precise identification of germplasm at the molecular level, molecular-assisted selection breeding, and the application of gene editing technology in broomcorn millet.
一个主要基因座SC9.1被鉴定出来，并被精细定位到一个92.68 Kb的区域，longmi004412被确定为调控黍稷种子褐色的因果基因。黍稷是一种谷类作物，在形态、农艺和产量相关性状上具有丰富的遗传变异。种子颜色的多样性是最显著的形态特征之一。然而，关于控制种子着色的遗传决定因素鲜有报道。在此，利用Longmi12和张778杂交产生的F和F群体来阐明种子颜色的遗传基础。对F、F和F后代的种子颜色进行统计分析，最终证明黍稷中褐色种子颜色由一个单显性基因座控制。通过混合分组分析法测序（BSA-seq），遗传控制基因座SC9.1初步定位在9号染色体上的32,175,878 - 44,281,406 bp区域。此外，利用260个隐性个体基因型进行精细定位，将SC9.1缩小到一个包含11个基因的92.68 kb区间。结合基因结构变异、转录组图谱和功能比较，longmi004412被确定为导致黍稷种子形成褐色的因果基因。此外，对516份材料中的longmi004412基因进行单倍型分析，以明确黍稷种子颜色的类型。这些发现为黍稷在分子水平上的种质精准鉴定、分子辅助选择育种以及基因编辑技术的应用奠定了基础。
