Jansen E H, Stephany R W
Vet Q. 1985 Jan;7(1):35-8. doi: 10.1080/01652176.1985.9693951.
In the period from August 1981 to April 1983, 10243 random samples of urine from slaughtered cattle were inspected for the presence of the stilbene derivatives diethylstilbestrol (DES), dienestrol (DE), and hexestrol (HEX). Fast screening of all samples was performed by radioimmunoassay (RIA) with prior clean-up column chromatography. In this screening, 216 samples were indicated for confirmatory analyses by combined high performance liquid chromatography-gas chromatography-mass spectrometry (HPLC-GCMS) on account of an immunochemical response equivalent to 1 microgram/1 DES or more. The presence of DES was confirmed in 184 samples, DE in 5 samples and HEX in 14 samples. In the remaining 13 samples no stilbene derivative could be confirmed. From the results in 1984, after the introduction of the Meat Inspection Act, it is concluded that the use of stilbene derivatives in cattle in the Netherlands has dropped almost to zero. Of 4558 samples investigated, only 6 (0.13%) were indicated as 'stilbene' positive. The presence of DES was confirmed in 2 samples and DE in 4 samples.
在1981年8月至1983年4月期间，对10243份屠宰牛尿液随机样本进行检查，以检测是否存在二苯乙烯衍生物己烯雌酚（DES）、dienestrol（DE）和己烷雌酚（HEX）。所有样本均先通过柱色谱净化，然后采用放射免疫分析（RIA）进行快速筛查。在此筛查中，有216份样本因免疫化学反应相当于1微克/1 DES或更高而被指定通过高效液相色谱-气相色谱-质谱联用（HPLC-GCMS）进行确证分析。184份样本中确认存在DES，5份样本中确认存在DE，14份样本中确认存在HEX。其余13份样本中未确认有二苯乙烯衍生物。根据1984年《肉类检验法》实施后的结果得出结论，荷兰牛中二苯乙烯衍生物的使用几乎已降至零。在调查的4558份样本中，只有6份（0.13%）被判定为“二苯乙烯”阳性。2份样本中确认存在DES，4份样本中确认存在DE。
