通过高效液相色谱分级分离和免疫化学检测鉴定牛尿液中的几种芪衍生物。

Identification of several stilbene derivatives in bovine urine by means of high performance liquid chromatographic fractionation and immunochemical detection.

作者信息

Jansen E H, van den Berg R H, van Blitterswijk H, Both-Miedema R, Stephany R W

出版信息

Vet Q. 1984 Jan;6(1):5-7. doi: 10.1080/01652176.1984.9693898.

DOI:10.1080/01652176.1984.9693898

Abstract

Radioimmunochemical detection (RIA) following fractionation of urine extracts via high performance liquid chromatography (HPLC) turned out to be a very specific method for the identification of stilbene derivatives in bovine urine. Combination of the high resolution of the HPLC with a specific RIA is a suitable method to discriminate between the presence of different stilbene derivatives like diethylstilboestrol (DES), dienoestrol (DE) and hexoestrol (HEX) or other unknown compounds interfering in the celite-RIA used in the screening. Using this screening method 8200 samples of bovine urine were investigated on the presence of stilbene derivatives of which 133 were classified as 'positive'. In 106 'positive' urines the presence of DES was shown and in 19 'positive' urines the presence of DE or HEX, using the method described in this report whereas in 8 'positive' urines an unknown immunochemical active compound was detected. During 1.5 year of comparative investigation no qualitative discrepancies occurred between the results of the HPLC-immunogram procedure and the final confirmation by high resolution gas chromatography-mass spectometry (GCMS).

摘要

通过高效液相色谱法（HPLC）对尿液提取物进行分级分离后进行放射免疫化学检测（RIA），结果表明这是一种鉴定牛尿液中二苯乙烯衍生物的非常特异的方法。将HPLC的高分辨率与特异性RIA相结合，是区分不同二苯乙烯衍生物（如己烯雌酚（DES）、双烯雌酚（DE）和己烷雌酚（HEX））或干扰筛选中使用的硅藻土-RIA的其他未知化合物存在与否的合适方法。使用这种筛选方法，对8200份牛尿液样本进行了二苯乙烯衍生物存在情况的调查，其中133份被归类为“阳性”。按照本报告所述方法，在106份“阳性”尿液中检测到了DES的存在，在19份“阳性”尿液中检测到了DE或HEX的存在，而在8份“阳性”尿液中检测到了一种未知的免疫化学活性化合物。在为期1.5年的对比研究中，HPLC免疫图谱程序的结果与高分辨率气相色谱-质谱法（GCMS）的最终确证结果之间未出现定性差异。