Yang Liupeng, Xia Yudie, Fei Chunmei, Shahzad Kashif, Niu Miao, Feng Juanjuan, Ma Jianjiang, Wang Xiaoping, Song Jikun, Feng Pan, Xu Siyuan, An Guoyong, Yu Jiwen
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China; State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, College of Life Sciences, Henan University, Kaifeng 475004, China.
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China.
Int J Biol Macromol. 2025 Mar;295:139529. doi: 10.1016/j.ijbiomac.2025.139529. Epub 2025 Jan 4.
Class III peroxidases (PRXs) play critical roles in plant growth and development by oxidizing various substrates with HO. Although many PRXs have been identified and their roles in biotic and abiotic stress responses have extensively investigated in plants. However, functional mechanisms of PRXs in seed development remain poorly understood. In this study, 14, 17, 9, and 13 PRX core genes were identified in Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum, and Gossypium raimondii, respectively. Phylogenetic analysis categorized PRXs core genes of cotton into five groups. Six of the GhPRX genes co-localized with quantitative trait loci (QTLs) associated with oil or seed size, and GhPRXR1-A showed significant high expression levels in developing ovules. Heterologous overexpression of GhPRXR1-A in Arabidopsis thaliana resulted in a significant increase in thousand seed weight (Col-0: 0.015 ± 0.00085 g vs. OE-GhPRXR1-A: 0.022 ± 0.0019 g). In addition, molecular mechanism assays revealed that GhPRXR1-A is directly activated by GhGATA1 and interacts with GhNFYC4, a transcription factor of the nuclear factor Y, C subunit family that has previously been reported to mediate seed development. Collectively, these findings suggest that GhPRXR1-A is a regulator of seed development in Arabidopsis and may have similar functional role in cotton seed contributing traits.
Ⅲ类过氧化物酶（PRXs）通过利用H₂O₂氧化各种底物在植物生长发育中发挥关键作用。尽管已经鉴定出许多PRXs，并且它们在植物生物和非生物胁迫响应中的作用也得到了广泛研究。然而，PRXs在种子发育中的功能机制仍知之甚少。在本研究中，分别在陆地棉、海岛棉、亚洲棉和雷蒙德氏棉中鉴定出14、17、9和13个PRX核心基因。系统发育分析将棉花的PRX核心基因分为五组。其中6个GhPRX基因与与油或种子大小相关的数量性状位点（QTLs）共定位，并且GhPRXR1-A在发育中的胚珠中表现出显著的高表达水平。在拟南芥中异源过表达GhPRXR1-A导致千粒重显著增加（Col-0：0.015±0.00085 g vs. OE-GhPRXR1-A：0.022±0.0019 g）。此外，分子机制分析表明，GhPRXR1-A直接被GhGATA1激活，并与GhNFYC4相互作用，GhNFYC4是核因子Y C亚基家族的转录因子，先前已报道其介导种子发育。总体而言，这些发现表明GhPRXR1-A是拟南芥种子发育的调节因子，并且可能在棉花种子相关性状中具有类似的功能作用。
