Fei Shengyi, Rule Blake D, Godwin Joshua S, Mobley C Brooks, Roberts Michael D, von Walden Ferdinand, Vechetti Ivan J
Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
School of Kinesiology, Auburn University, Auburn, Alabama, USA.
Physiol Rep. 2025 Jan;13(1):e70166. doi: 10.14814/phy2.70166.
MicroRNAs (miRNAs) are small, noncoding RNAs that play a critical role in regulating gene expression post-transcriptionally. They are involved in various developmental and physiological processes, and their dysregulation is linked to various diseases. Skeletal muscle-specific miRNAs, including miR-1, play a crucial role in the development and maintenance of skeletal muscle. It has been demonstrated that the expression of miR-1 decreases by approximately 50% in response to hypertrophic stimuli, suggesting its potential involvement in muscle hypertrophy. In our study, we hypothesize that reduction of miR-1 levels is necessary for skeletal muscle growth due to its interaction to essential pro-growth genes. Promoting a smaller reduction of miR-1 levels, we observed a blunted hypertrophic response in mice undergoing a murine model of muscle hypertrophy. In addition, our results suggest that miR-1 inhibits the expression of Itm2a, a membrane-related protein, as potential miR-1-related candidate for skeletal muscle hypertrophy. While the exact mechanism in muscle hypertrophy has not been identified, our results suggest that miR-1-regulated membrane proteins are important for skeletal muscle hypertrophy.
微小RNA（miRNA）是一类小的非编码RNA，在转录后调控基因表达中发挥关键作用。它们参与各种发育和生理过程，其失调与多种疾病相关。包括miR-1在内的骨骼肌特异性miRNA在骨骼肌的发育和维持中起关键作用。已经证明，在肥大刺激下，miR-1的表达下降约50%，这表明其可能参与肌肉肥大。在我们的研究中，我们假设由于miR-1与重要的促生长基因相互作用，其水平降低对于骨骼肌生长是必要的。在促进miR-1水平较小程度降低的情况下，我们在经历肌肉肥大小鼠模型的小鼠中观察到肥大反应减弱。此外，我们的结果表明，miR-1抑制Itm2a（一种膜相关蛋白）的表达，Itm2a是骨骼肌肥大潜在的miR-1相关候选蛋白。虽然肌肉肥大的确切机制尚未确定，但我们的结果表明，miR-1调控的膜蛋白对骨骼肌肥大很重要。
