Li Lele, Ge Shengyu, Wang Yang, Zhu Heyun, Feng Bo
School of Pharmacy, Jilin Medical University, Jilin, 132013, Jilin, China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130117, Jilin, China.
Sci Rep. 2025 Jan 6;15(1):937. doi: 10.1038/s41598-024-84028-5.
Scorpion is a commonly used drug in traditional Chinese medicine for treating epilepsy, although the exact mechanisms are not yet fully understood. This study aimed to compare the treatment effects of Scorpion water extract (SWE) and Scorpion ethanol extract (SEE) on mice with pentetrazole-induced epilepsy and investigate the possible mechanisms through metabolomics methods. A pentetrazole-induced epileptic mice model was used to assess the corrective effects of SWE and SEE. Untargeted metabolomics, utilizing UPLC-Q-TOF/MS, was employed to analyze the metabolic profiles of mice and identify metabolic changes following scorpion treatment. The results revealed that only SWE showed therapeutic effects in epileptic mice. Metabolomics analysis demonstrated significant alterations in metabolic signatures between the pentetrazole-induced epileptic mice and SWE groups. By utilizing orthogonal partial least squares discrimination analysis, 44 and 108 potential biomarkers in mouse serum were identified in positive and negative ion modes, respectively. Differential metabolites related to epilepsy were then used to pinpoint relevant pathways in epileptic mice, such as linoleic acid metabolism, biosynthesis of unsaturated fatty acids, glycerophospholipid metabolism, and ether lipid metabolism. In conclusion, this study highlights the corrective effects of Scorpion on epileptic mice and provides insight into the underlying metabolic pathways involved in its efficacy.
全蝎是中医治疗癫痫常用的药物，但其确切机制尚未完全明确。本研究旨在比较全蝎水提取物（SWE）和全蝎乙醇提取物（SEE）对戊四氮诱导癫痫小鼠的治疗效果，并通过代谢组学方法探讨其可能的作用机制。采用戊四氮诱导的癫痫小鼠模型评估SWE和SEE的治疗效果。利用超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-Q-TOF/MS）进行非靶向代谢组学分析，以分析小鼠的代谢谱并确定全蝎治疗后的代谢变化。结果显示，仅SWE对癫痫小鼠具有治疗作用。代谢组学分析表明，戊四氮诱导的癫痫小鼠与SWE组之间的代谢特征存在显著差异。通过正交偏最小二乘法判别分析，在正离子模式和负离子模式下分别在小鼠血清中鉴定出44种和108种潜在生物标志物。然后利用与癫痫相关的差异代谢物来确定癫痫小鼠中的相关代谢途径，如亚油酸代谢、不饱和脂肪酸生物合成、甘油磷脂代谢和醚脂代谢。总之，本研究突出了全蝎对癫痫小鼠的治疗作用，并深入了解了其疗效所涉及的潜在代谢途径。
