Divya Priya A, Martin Asha
Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570020, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India.
Sci Rep. 2025 Jan 6;15(1):945. doi: 10.1038/s41598-024-84800-7.
Rhodomyrtus tomentosa fruits, endemic to the Western Ghats were analyzed for its free, bound and esterified phenolics by Ultra High Performance Liquid Chromatography-Mass Spectrometry. Overall, twenty-nine phenolic compounds were identified, amongst them 18 were detected in this fruit for the first time. Gallic acid (80.44 ± 8.74 mg/100 g) and ellagic acid (107.47 ± 7.28 mg/100 g) were the most prominent ones found in the bound phenolic fraction and gallic acid (103.76 ± 6.34 mg/100 g) in the esterified phenolic fraction of the fruit, respectively. Total Phenolic content was found to be highest in bound phenolics (7.09 ± 0.17 mg Gallic acid equivalent/g). The antioxidant and antimicrobial activities of the three extracts namely free, bound and esterified phenolic fruit fractions have been analyzed. Bound phenolics exhibited the highest antioxidant potential (DPPH-15.63 ± 0.86; ABTS-34.73 ± 0.07; FRAP-17.89 ± 0.27 mg/g Ascorbic acid equivalent). The bound phenolics showed good antimicrobial activity against Bacillus cereus, Staphylococcus aureus and Escherichia coli with a MIC of 0.156, 0.625 and 1.25 mg/mL respectively. The exploration of phenolic compounds in Indian variety of Rhodomyrtus tomentosa fruits may provide useful insights on its utilization as a functional food ingredient.
对西高止山脉特有的桃金娘科蒲桃果实中的游离、结合和酯化酚类物质进行了超高效液相色谱-质谱分析。总体而言，共鉴定出29种酚类化合物，其中18种是首次在该果实中检测到。没食子酸（80.44±8.74毫克/100克）和鞣花酸（107.47±7.28毫克/100克）分别是结合酚类组分中最主要的成分，没食子酸（103.76±6.34毫克/100克）是该果实酯化酚类组分中最主要的成分。发现结合酚类中的总酚含量最高（7.09±0.17毫克没食子酸当量/克）。对游离、结合和酯化酚类果实组分这三种提取物的抗氧化和抗菌活性进行了分析。结合酚类表现出最高的抗氧化潜力（DPPH-15.63±0.86；ABTS-34.73±0.07；FRAP-17.89±0.27毫克/克抗坏血酸当量）。结合酚类对蜡样芽孢杆菌、金黄色葡萄球菌和大肠杆菌表现出良好的抗菌活性，其最低抑菌浓度分别为0.156、0.625和1.25毫克/毫升。对印度品种桃金娘科蒲桃果实中酚类化合物的探索可能为其作为功能性食品成分的利用提供有用的见解。
