School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Medicinal Plants Innovation Center, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Sci Rep. 2022 Feb 9;12(1):2225. doi: 10.1038/s41598-022-06358-6.
The essential oil of Elsholtzia beddomei C. B. Clarke ex Hook. f. was investigated for its chemical composition and tested for antioxidant and antimicrobial activities. The E. beddomei essential oil was extracted using hydrodistillation for 4 h (yield of 1.38% w/w). Forty-three volatile compounds were identified in the E. beddomei essential oil, including linalool (83.67%), perillaldehyde (4.68%), neral (3.68%), perillene (1.65%), E-caryophyllene (1.55%), and α-zingiberene (1.06%) as the major compounds. The antioxidant activity of the E. beddomei essential oil was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation scavenging activity. The IC values calculated using the DPPH and ABTS methods were 148.31 and 172.22 µg/mL, respectively. In addition, using disc diffusion and broth microdilution methods, the antimicrobial activities of the E. beddomei essential oil against Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, and Candida albicans were evaluated. The E. beddomei essential oil possessed an inhibitory effect with the minimum inhibitory concentration in the range of 31.25-250.00 µg/mL among these pathogens. The results indicated that E. beddomei essential oil is an alternative raw material of food, and medicinal products for use in pharmaceutical applications.
采用水蒸汽蒸馏法提取圆叶当归（Elsholtzia beddomei C. B. Clarke ex Hook. f.）挥发油，提取时间为 4 h，出油率为 1.38%（w/w）。采用气相色谱-质谱联用技术对挥发油成分进行分析鉴定，共鉴定出 43 种化合物，主要成分是芳樟醇（83.67%）、紫苏醛（4.68%）、柠檬醛（3.68%）、紫苏烯（1.65%）、石竹烯（1.55%）和姜烯（1.06%）。采用 1，1-二苯基-2-三硝基苯肼（DPPH）自由基法和 2，2-连氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子清除法测定挥发油的抗氧化活性，IC 50 值分别为 148.31 和 172.22 μg/mL。采用纸片扩散法和微量稀释法测定挥发油对大肠杆菌、铜绿假单胞菌、产气肠杆菌、金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌和白色念珠菌的抑菌活性，其最低抑菌浓度（MIC）值在 31.25-250.00 μg/mL 之间。结果表明，圆叶当归挥发油具有一定的抗氧化和抑菌活性，是一种有潜力的天然抗氧化剂和抗菌剂，可作为食品、药品的开发资源。
