Matsumura Yasufumi, Yamamoto Masaki, Tsuda Yusuke, Shinohara Koh, Tsuchido Yasuhiro, Yukawa Satomi, Noguchi Taro, Takayama Kazuo, Nagao Miki
Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Sci Rep. 2025 Jan 6;15(1):924. doi: 10.1038/s41598-024-85068-7.
The seasonality and epidemiology of viral acute respiratory infections (ARIs) have changed since the coronavirus disease 2019 pandemic. However, molecular-based ARI surveillance has not been conducted in Japan. We developed a regional surveillance program to define the local epidemiology of ARIs. Between December 2023 and March 2024, 2,992 upper respiratory samples collected from patients suspected of having ARIs at five facilities in Kyoto City, Japan, were tested for SARS-CoV-2, influenza virus, and respiratory syncytial virus (RSV) using RT‒PCR. Samples negative for these viruses were randomly selected for testing with the FilmArray Respiratory Panel, and the detection rates of other viruses were estimated. SARS-CoV-2, influenza virus, and RSV were detected in 598 (20.3%), 165 (5.6%), and 40 (1.4%) of the 2,949 samples with valid RT‒PCR results, respectively. The most prevalent viruses in the < 6, 6-17, 18-64, and ≥ 65 year age groups were rhinovirus/enterovirus, RSV, and SARS-CoV-2; influenza virus, seasonal coronavirus, and rhinovirus/enterovirus; SARS-CoV-2, seasonal coronavirus, and influenza virus; and SARS-CoV-2, seasonal coronavirus, and influenza virus, respectively. Significant differences in the detection rates of these viruses were detected between the age groups. This study highlights the importance of age-stratified molecular-based surveillance for a comprehensive understanding of the epidemiology of ARIs.
自2019年冠状病毒病大流行以来，病毒性急性呼吸道感染（ARI）的季节性和流行病学发生了变化。然而，日本尚未开展基于分子的ARI监测。我们制定了一项区域监测计划，以确定ARI的当地流行病学情况。在2023年12月至2024年3月期间，对从日本京都市五家医疗机构疑似患有ARI的患者采集的2992份上呼吸道样本，使用逆转录-聚合酶链反应（RT-PCR）检测了严重急性呼吸综合征冠状病毒2（SARS-CoV-2）、流感病毒和呼吸道合胞病毒（RSV）。对这些病毒检测呈阴性的样本随机选择使用FilmArray呼吸道病原体检测板进行检测，并估计其他病毒的检出率。在2949份RT-PCR结果有效的样本中，分别检测到SARS-CoV-2、流感病毒和RSV的样本有598份（20.3%）、165份（5.6%）和40份（1.4%）。在<6岁、6-17岁、18-64岁和≥65岁年龄组中，最常见的病毒分别是鼻病毒/肠道病毒、RSV和SARS-CoV-2；流感病毒、季节性冠状病毒和鼻病毒/肠道病毒；SARS-CoV-2、季节性冠状病毒和流感病毒；以及SARS-CoV-2、季节性冠状病毒和流感病毒。这些病毒的检出率在不同年龄组之间存在显著差异。本研究强调了基于分子的年龄分层监测对于全面了解ARI流行病学的重要性。
