Yuan Wenhui, Yang Deqiang, Xu Zhipeng, Liu Yunwei, Li Xiangquan, Ding Changhong, Wang Zhibin
Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
Curr Microbiol. 2025 Jan 6;82(2):73. doi: 10.1007/s00284-024-04054-4.
Comprehending the interplay between the microbial communities of bulk soil (BS) and rhizosphere soil (RS) holds crucial significance in maintaining soil health and fertility, as well as enhancing crop quality. Our research focused on examining these microbial communities in BS and RS of Acanthopanax senticosus, along with their correlation with soil nutrients, across three distinct habitats in Yichun, Heilongjiang Province. To achieve this, we employed high-throughput sequencing technology, specifically targeting the 16S and amplicon regions. The results showed that there were significant differences in soil nutrients, microbial diversity and composition between BS and RS in different habitats. Available phosphorus (AP), total nitrogen (TN), ammonium-nitrogen (NH-N) and available potassium (AK) content in RS were higher than that in BS, but TP content was opposite. The Mantel experiment's findings revealed that the soil physicochemical attributes exerted a more significant impact on the microbial community present in RS compared to its influence in BS. Redundancy analysis (RDA) indicated that the bacterial community of BS was affected by SMC and TP. The fungal structure of BS was affected by soil moisture content (SMC), AP, AK (P < 0.01) and TN (P < 0.05). The fungal structure of RS was affected by soil organic carbon (SOC), AP and AK. A noteworthy inverse relationship was observed between Actinomycetes and SMC as well as AP. In conclusion, these results extend the understanding of soil microbial community of A. senticosus and provide a preliminary understanding of the effects of habitat changes caused by human activities on A. senticosus.
了解大田土壤（BS）和根际土壤（RS）微生物群落之间的相互作用对于维持土壤健康和肥力以及提高作物品质至关重要。我们的研究聚焦于黑龙江省伊春市三个不同生境下刺五加大田土壤和根际土壤中的这些微生物群落，以及它们与土壤养分的相关性。为此，我们采用了高通量测序技术，特别针对16S和扩增子区域。结果表明，不同生境下大田土壤和根际土壤在土壤养分、微生物多样性和组成方面存在显著差异。根际土壤中有效磷（AP）、全氮（TN）、铵态氮（NH-N）和速效钾（AK）含量高于大田土壤，但总磷（TP）含量则相反。Mantel实验结果表明，土壤理化属性对根际土壤中微生物群落的影响比对大田土壤中微生物群落的影响更大。冗余分析（RDA）表明，大田土壤细菌群落受土壤水分含量（SMC）和总磷影响。大田土壤真菌结构受土壤水分含量、有效磷、速效钾（P < 0.01）和全氮（P < 0.05）影响。根际土壤真菌结构受土壤有机碳（SOC）、有效磷和速效钾影响。观察到放线菌与土壤水分含量和有效磷之间存在显著的负相关关系。总之，这些结果扩展了对刺五加土壤微生物群落的认识，并初步了解了人类活动引起的生境变化对刺五加的影响。
